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Literature summary for 3.2.1.4 extracted from

  • Molhoj, M.; Ulvskov, P.; Dal Degan, F.
    Characterization of a functional soluble form of a Brassica napus membrane-anchored endo-1,4-beta-glucanase heterologously expressed in Pichia pastoris (2001), Plant Physiol., 127, 674-684.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.4

Cloned(Commentary)

Cloned (Comment) Organism
N-terminally truncated mutant DELTA1-90 is expressed as a soluble protein in Pichia pastoris Brassica napus

Protein Variants

Protein Variants Comment Organism
DELTA1-90 expressed as a soluble protein in Pichia pastoris, the wild-type enzyme is anchored to membrane Brassica napus

Inhibitors

Inhibitors Comment Organism Structure
EDTA 5 mM, complete inhibition of mutant enzyme DELTA1-90 Brassica napus
Mg2+ above 10 mM Brassica napus
Zn2+ above 5 mM Brassica napus

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane wild-type enzyme is anchored to membrane Brassica napus 16020
-
soluble the N-terminally truncated mutant DELTA1-90 is expressed as a soluble protein in Pichia pastoris Brassica napus
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activity of mutant enzyme DELTA1-90 is enhances in a concentration-dependent manner up to about 40 mM Brassica napus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
130000
-
 x * 130000, mutant enzyme DELTA1-90, SDS-PAGE Brassica napus

Organism

Organism UniProt Comment Textmining
Brassica napus Q9STD9
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein MW detected by SDS-PAGE is 130000 Da, after removal of N-glycans the MW is 60000 Da Brassica napus

Purification (Commentary)

Purification (Comment) Organism
mutant enzyme DELTA1-90 Brassica napus

Source Tissue

Source Tissue Comment Organism Textmining
culture medium
-
 Brassica napus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
avicel + H2O
-
 Brassica napus ?
-
 ?
carboxymethylcellulose + H2O activity with carbomethylcellulose with approxomately 4 carboxymethyl groups per 10 anhydro-glucose units is about 3.5 times better than that with carbomethylcellulose with approximately 8 carboxymethyl groups Brassica napus ?
-
 ?
additional information no activity with tamarind xyloglucan, barley (1-3)(1-4)-beta-D-glucan, wheat arabinoxylan or birchwood xylan Brassica napus ?
-
 ?
phosphoric acid-swollen cellulose + H2O
-
 Brassica napus ?
-
 ?

Subunits

Subunits Comment Organism
? x * 130000, mutant enzyme DELTA1-90, SDS-PAGE Brassica napus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 mutant enzyme DELTA1-90, hydrolysis of carbomethylcellulose with approximately 4 carboxymethyl groups per 10 anhydro-glucose units Brassica napus

pH Range

pH Minimum pH Maximum Comment Organism
5.8 6.4 pH 5.8: about 55% of maximal activity, pH 6.4: about 55% of maximal activity, mutant enzyme DELTA1-90, hydrolysis of carbomethylcellulose with approxomately 4 carboxymethyl groups per 10 anhydro-glucose units Brassica napus