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Literature summary for 3.2.1.4 extracted from

  • Rahman, M.; Bhuiyan, S.H.; Nirasawa, S.; Kitaoka, M.; Hayashi, K.
    Characterization of an endo-beta-1,4-glucanase of Thermotoga maritima expressed in Escherichia coli (2002), J. Appl. Glycosci., 49, 487-495.
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Thermotoga maritima

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.24
-
p-nitrophenyl-beta-D-cellopentaoside
-
Thermotoga maritima
0.25
-
p-nitrophenyl-beta-D-cellotetraoside
-
Thermotoga maritima

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38000
-
x * 38000, SDS-PAGE Thermotoga maritima

Organism

Organism UniProt Comment Textmining
Thermotoga maritima Q9X273
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Thermotoga maritima

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
barley glucan + H2O the enzyme hydrolyzes mixed linked beta-(1-3)(1-4) substrates such as barley glucan and lichenan more strongly than carboxymethylcellulose Thermotoga maritima ?
-
?
carboxymethylcellulose + H2O the enzyme hydrolyzes mixed linked beta-(1-3)(1-4) substrates such as barley glucan and lichenan more strongly than carboxymethylcellulose Thermotoga maritima ?
-
?
lichenan + H2O the enzyme hydrolyzes mixed linked beta-(1-3)(1-4) substrates such as barley glucan and lichenan more strongly than carboxymethylcellulose Thermotoga maritima ?
-
?
additional information transglycosylation activity with smaller soluble cellooligosaccharides Thermotoga maritima ?
-
?
p-nitrophenyl-beta-D-cellopentaoside + H2O
-
Thermotoga maritima ?
-
?
p-nitrophenyl-beta-D-cellotetraoside + H2O
-
Thermotoga maritima ?
-
?

Subunits

Subunits Comment Organism
? x * 38000, SDS-PAGE Thermotoga maritima

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
90
-
-
Thermotoga maritima

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
85
-
stable up to Thermotoga maritima

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.6
-
-
Thermotoga maritima

pH Range

pH Minimum pH Maximum Comment Organism
4 9.5 active in the range Thermotoga maritima