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Literature summary for 3.2.1.4 extracted from

  • Ohara, H.; Noguchi, J.; Karita, S.; Kimura, T.; Sakka, K.; Ohmiya, K.
    Sequence of egV and properties of EgV, a Ruminococcus albus endoglucanase containing a dockerin domain (2000), Biosci. Biotechnol. Biochem., 64, 80-88.
    View publication on PubMed
Search for more literature for 3.2.1.4

Cloned(Commentary)

Cloned (Comment) Organism
truncated enzyme form purified from recombinant Escherichia coli Ruminococcus albus

Localization

Localization Comment Organism GeneOntology No. Textmining
cell surface
-
 Ruminococcus albus 9986
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
 x * 42000, truncated enzyme form expressed in Escherichia coli Ruminococcus albus
67103
-
 x * 67103, calculation from nucleotide sequence Ruminococcus albus

Organism

Organism UniProt Comment Textmining
Ruminococcus albus Q9LCC5 F-40
-
Ruminococcus albus F-40 Q9LCC5 F-40
-

Purification (Commentary)

Purification (Comment) Organism
-
 Ruminococcus albus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acid-swollen cellulose + H2O activity of truncated enzyme form Ruminococcus albus ?
-
 ?
acid-swollen cellulose + H2O activity of truncated enzyme form Ruminococcus albus F-40 ?
-
 ?
carboxymethyl cellulose + H2O activity of truncated enzyme form Ruminococcus albus ?
-
 ?
carboxymethyl cellulose + H2O activity of truncated enzyme form Ruminococcus albus F-40 ?
-
 ?
lichenan + H2O activity of truncated enzyme form Ruminococcus albus ?
-
 ?
lichenan + H2O activity of truncated enzyme form Ruminococcus albus F-40 ?
-
 ?
xylan + H2O activity of truncated enzyme form Ruminococcus albus ?
-
 ?
xylan + H2O activity of truncated enzyme form Ruminococcus albus F-40 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 42000, truncated enzyme form expressed in Escherichia coli Ruminococcus albus
? x * 67103, calculation from nucleotide sequence Ruminococcus albus
More modular enzyme composed of a catalytic domain of family 5 of glycosyl hydrolases, a domain of unknown function, and a dockerin domain responsible for cellulosome assembly. The enzyme is a component of the cellulosome Ruminococcus albus

Synonyms

Synonyms Comment Organism
EgV
-
 Ruminococcus albus
endoglucanase V
-
 Ruminococcus albus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
 truncated enzyme form Ruminococcus albus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 1 0 min, stable Ruminococcus albus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 truncated enzyme form Ruminococcus albus

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 9 37°C, 30 min, stable Ruminococcus albus