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Literature summary for 3.2.1.4 extracted from
- Cloning and characterization of thermostable endoglucanase (Cel8Y) from the hyperthermophilic Aquifex aeolicus VF5 (2000), Biochem. Biophys. Res. Commun., 279, 420-426.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli XL1-Blue | Aquifex aeolicus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 36750 | - |
x * 36750, SDS-PAGE | Aquifex aeolicus |
| 38831 | - |
x * 38831, calculation from nucleotide sequence | Aquifex aeolicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aquifex aeolicus | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| carboxymethylcellulose + H2O | - |
Aquifex aeolicus | ? | - |
? |  |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 36750, SDS-PAGE | Aquifex aeolicus |
| ? | x * 38831, calculation from nucleotide sequence | Aquifex aeolicus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Cel8Y | - |
Aquifex aeolicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
hydrolysis of carboxymethylcellulose | Aquifex aeolicus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 100 | 40°C: about 60% of maximal activity, 100°C: about 85% of maximal activity, hydrolysis of carboxymethylcellulose | Aquifex aeolicus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
2 h, stable | Aquifex aeolicus |
| 90 | - |
half-life: 4 h | Aquifex aeolicus |
| 100 | - |
half-life: 2 h | Aquifex aeolicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
hydrolysis of carboxymethylcellulose | Aquifex aeolicus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 8 | about 70% of maximal activity at pH 5.0 and pH 8.0, hydrolysis of carboxymethylcellulose | Aquifex aeolicus |
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