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Literature summary for 3.2.1.4 extracted from
- Purification and properties of a wall-bound endo-1,4-beta-glucanase from suspension-cultured poplar cells (1995), Plant Cell Physiol., 36, 607-614.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Cysteine hydrochloride | strong stimulation | Populus x canadensis |  |
| dithiothreitol | slight activation | Populus x canadensis | |
| glutathione | slightly enhanced | Populus x canadensis | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | 1 mM CuCl2, 55% inhibition | Populus x canadensis | |
| EDTA | 1 mM, 55% inhibition | Populus x canadensis | |
| Fe2+ | 1 mM FeCl2, 13% inhibition | Populus x canadensis | |
| Hg2+ | 1 mM HgCl2, complete inhibition | Populus x canadensis | |
| Mn2+ | 1 mM MnCl2, 18% inhibition | Populus x canadensis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Populus x canadensis |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cell wall | bound to | Populus x canadensis | 5618 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | - |
Populus x canadensis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 47000 | - |
1 * 47000, SDS-PAGE | Populus x canadensis |
| 48000 | - |
gel filtration | Populus x canadensis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Populus x canadensis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Populus x canadensis |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell suspension culture | - |
Populus x canadensis | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Populus x canadensis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| carboxymethylcellulose + H2O | - |
Populus x canadensis | additional information | - |
? | |
| cellulose + H2O | acid-swollen | Populus x canadensis | ? | - |
? | |
| lichenan + H2O | - |
Populus x canadensis | ? | - |
? | |
| xylan + H2O | - |
Populus x canadensis | additional information | - |
? | |
| xyloglucan + H2O | - |
Populus x canadensis | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 47000, SDS-PAGE | Populus x canadensis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
- |
Populus x canadensis |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 7.5 | 4°C, stable for at least 1 month in 10 mM phosphate buffer | Populus x canadensis |
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Release 2023.1 (January 2023)
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