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Literature summary for 3.2.1.4 extracted from
- Enzymatic studies on a cellulase system of Trichoderma viride. II. Purification and properties of two cellulases (1975), J. Biochem., 77, 33-42.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | 1 mM, partial inhibition | Trichoderma viride |  |
| Cu2+ | 1 mM, partial inhibition | Trichoderma viride | |
| Hg2+ | 1 mM, complete inhibition | Trichoderma viride | |
| KMnO4 | 0.1 mM, complete inhibition of cellulase II-A | Trichoderma viride | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 30000 | - |
cellulase II-A, gel filtration | Trichoderma viride |
| 43000 | - |
cellulase II-B, gel filtration | Trichoderma viride |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Trichoderma viride | - |
cellulase II-A and II-B | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | cellulase II-A contains 12% carbohydrate, cellulase II-B contains 14% carbohydrate | Trichoderma viride |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| cellulase II-A and II-B | Trichoderma viride |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
cellulase II-B | Trichoderma viride |
| 60 | - |
cellulase IIa | Trichoderma viride |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
2 h, cellulase II-B is stable over the pH-range 4.5-6.0, cellulase II-A is unstable, except at pH 5.5-6.0 | Trichoderma viride |
| 100 | - |
10 min, cellulase II-A retains 27% of the original activity, cellulase II-B retains 41% of the original activity | Trichoderma viride |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 5 | - |
Trichoderma viride |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 6 | 45°C, 2 h, cellulase II-B is stable | Trichoderma viride |
| 5.5 | 6 | 5°C, 2 h, cellulase II-A is stable | Trichoderma viride |
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Release 2023.1 (January 2023)
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