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Literature summary for 3.2.1.4 extracted from
- Isolation, crystallization and properties of a new cellulase of Clostridium thermocellum overproduced in Escherichia coli (1986), Bio/Technology, 4, 896-900.
No PubMed abstract available
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Acetivibrio thermocellus |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
- |
Acetivibrio thermocellus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 65000 | - |
equilibrium sedimentation | Acetivibrio thermocellus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Acetivibrio thermocellus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Acetivibrio thermocellus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 428 | - |
recombinant enzyme from granules produced by Escherichia coli | Acetivibrio thermocellus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| avicel + H2O | no hydrolysis | Acetivibrio thermocellus | ? | - |
? |  |
| cellulose + H2O | Walseth cellulose shows 1.1% of the activity with carboxymethylcellulose | Acetivibrio thermocellus | ? | - |
? | |
| lichenan + H2O | 6% of the activity with carboxymethylcellulose | Acetivibrio thermocellus | ? | - |
? | |
| additional information | no activity with p-nitrophenyl-beta-D-glucoside | Acetivibrio thermocellus | ? | - |
? | |
| additional information | no activity with cellobiose | Acetivibrio thermocellus | ? | - |
? | |
| xylan + H2O | no activity | Acetivibrio thermocellus | additional information | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
denaturation above | Acetivibrio thermocellus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
hydrolysis of carboxymethylcellulose | Acetivibrio thermocellus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4.8 | 8.2 | more than 50% of maximal activity at pH 4.8 and at pH 8.2 | Acetivibrio thermocellus |
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