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Literature summary for 3.2.1.4 extracted from

  • Yoshigi, N.; Taniguchi, H.; Sasaki, T.
    Purification and properties of a new endo-cellulase from Robillarda sp. Y-20 (1988), Agric. Biol. Chem., 52, 1389-1396.
No PubMed abstract available
Search for more literature for 3.2.1.4

Inhibitors

Inhibitors Comment Organism Structure
Ba2+
-
 Robillarda sp.
Cu2+
-
 Aspergillus niger
Cu2+
-
 Robillarda sp.
Cu2+
-
 Trichoderma viride
Fe3+
-
 Aspergillus niger
Fe3+
-
 Robillarda sp.
Hg2+
-
 Aspergillus niger
Hg2+
-
 Robillarda sp.
Hg2+
-
 Trichoderma viride
Mg2+ weak inhibition Robillarda sp.
N-bromosuccinimide
-
 Aspergillus niger
N-bromosuccinimide
-
 Robillarda sp.
Zn2+
-
 Robillarda sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Aspergillus niger
additional information
-
 additional information
-
 Trichoderma viride
additional information
-
 additional information
-
 Robillarda sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
56000
-
 x * 56000, SDS-PAGE Robillarda sp.

Organism

Organism UniProt Comment Textmining
Aspergillus niger
-
-
-
Robillarda sp.
-
 Y-20
-
Robillarda sp. Y-20
-
 Y-20
-
Trichoderma viride
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Aspergillus niger
glycoprotein
-
 Trichoderma viride
glycoprotein contains 10.5% carbohydrate Robillarda sp.

Purification (Commentary)

Purification (Comment) Organism
CMCase I Robillarda sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
carboxymethylcellulose + H2O
-
 Aspergillus niger additional information
-
 ?
carboxymethylcellulose + H2O
-
 Trichoderma viride additional information
-
 ?
carboxymethylcellulose + H2O
-
 Robillarda sp. additional information
-
 ?
carboxymethylcellulose + H2O
-
 Robillarda sp. Y-20 additional information
-
 ?
cellohexaose + H2O
-
 Aspergillus niger additional information
-
 ?
cellohexaose + H2O
-
 Trichoderma viride additional information
-
 ?
cellohexaose + H2O
-
 Robillarda sp. additional information
-
 ?
cellopentaose transfer reaction Robillarda sp. cellohexaose + celloheptaose
-
 ?
cellopentaose + H2O
-
 Aspergillus niger additional information
-
 ?
cellopentaose + H2O
-
 Trichoderma viride additional information
-
 ?
cellopentaose + H2O
-
 Robillarda sp. additional information
-
 ?
cellotetraose transfer reaction Robillarda sp. cellopentaose + cellohexaose
-
 ?
cellotetraose transfer reaction Robillarda sp. Y-20 cellopentaose + cellohexaose
-
 ?
cellotetraose + H2O
-
 Aspergillus niger 2 cellobiose
-
 ?
cellotetraose + H2O
-
 Trichoderma viride 2 cellobiose
-
 ?
cellotetraose + H2O
-
 Robillarda sp. 2 cellobiose predominant product ?
cellotetraose + H2O
-
 Robillarda sp. Y-20 2 cellobiose predominant product ?
cellotriose transfer reaction Robillarda sp. cellopentaose + cellotetraose
-
 ?
cellotriose transfer reaction Robillarda sp. Y-20 cellopentaose + cellotetraose
-
 ?
cellotriose + H2O
-
 Aspergillus niger D-glucose + cellobiose
-
 ?
cellotriose + H2O
-
 Trichoderma viride D-glucose + cellobiose
-
 ?
cellotriose + H2O
-
 Robillarda sp. D-glucose + cellobiose predominant product ?
cellotriose + H2O
-
 Robillarda sp. Y-20 D-glucose + cellobiose predominant product ?

Subunits

Subunits Comment Organism
?
-
 Aspergillus niger
?
-
 Trichoderma viride
? x * 56000, SDS-PAGE Robillarda sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Trichoderma viride
60
-
 CMCase I Robillarda sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
37 70 about 35% of maximal activity at 37°C and at 70°C Robillarda sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 30 min, CMCase I, stable below Robillarda sp.
60
-
-
 Aspergillus niger
60
-
 pH 5.0, 30 min, CMCase I, 50% loss of activity Robillarda sp.
70
-
-
 Aspergillus niger
70
-
 pH 5.0, 30 min, CMCase I, complete inactivation Robillarda sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Aspergillus niger
5
-
-
 Trichoderma viride
5
-
 CMCase I Robillarda sp.

pH Range

pH Minimum pH Maximum Comment Organism
4 6.5 about 75% of maximal activity at pH 4.0 and at pH 6.5, CMCase I Robillarda sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
3.5
-
 rapid inactivation below, CMCase I Robillarda sp.
4 7 37°C, 1 h, CMCase I, stable Robillarda sp.