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Literature summary for 3.2.1.4 extracted from
- Characterization of a Streptomyces rochei endoglucanase (1996), Ann. N. Y. Acad. Sci., 782, 173-181.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli in a correctly processed form | Streptomyces rochei |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 29000 | - |
x * 29000, catalytic domain, SDS-PAGE | Streptomyces rochei |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces rochei | - |
A2 | - |
| Streptomyces rochei A2 | - |
A2 | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 64.8 | - |
catalytic domain expressed in Escherichia coli | Streptomyces rochei |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, catalytic domain is stable for at least 1 week | Streptomyces rochei |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cellulose + H2O | - |
Streptomyces rochei | ? | - |
? |  |
| cellulose + H2O | - |
Streptomyces rochei A2 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 29000, catalytic domain, SDS-PAGE | Streptomyces rochei |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
catalytic domain | Streptomyces rochei |
| 65 | - |
whole enzyme | Streptomyces rochei |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | 80 | 37°C: about 50% of maximal activity of the catalytic domain and of the whole enzyme, 80°C: about 30% of maximal activity of the catalytic domain, about 45% of maximal activity of the whole enzyme | Streptomyces rochei |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
30 min, more than 50% loss of activity of the catalytic domain and of the whole enzyme | Streptomyces rochei |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
catalytic domain | Streptomyces rochei |
| 6 | - |
whole enzyme | Streptomyces rochei |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 8 | pH 4.0: about 25% of maximal activity of the whole enzyme about 45% of maximal activity of the catalytic domain, pH 8.0: about 45% of maximal activity of the catalytic domain, about 55% of maximal activity of the whole enzyme | Streptomyces rochei |
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