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Literature summary for 3.2.1.4 extracted from

  • Ng, T.K.; Zeikus, J.G.
    Endoglucanase from Clostridium thermocellum (1988), Methods Enzymol., 160, 351-355.
No PubMed abstract available

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
83000
-
endoglucanase I, equilibrium sedimentation Acetivibrio thermocellus
94000
-
endoglucanase I, non-denaturing PAGE Acetivibrio thermocellus

Organism

Organism UniProt Comment Textmining
Acetivibrio thermocellus
-
production of several endoglucanases, which are part of a cellulase complex termed cellulosome
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein endoglucanase I contains 11% carbohydrate Acetivibrio thermocellus

Purification (Commentary)

Purification (Comment) Organism
endoglucanase I Acetivibrio thermocellus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
65.1
-
endoglucanase I Acetivibrio thermocellus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
avicel + H2O endoglucanase I show very low activity Acetivibrio thermocellus ?
-
?
carboxymethylcellulose + H2O
-
Acetivibrio thermocellus additional information
-
?
cellulose + H2O endoglucanase I shows very low activity with acid-swollen cellulose Acetivibrio thermocellus ?
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
62
-
-
Acetivibrio thermocellus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.2
-
-
Acetivibrio thermocellus