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Literature summary for 3.2.1.4 extracted from
- Purification and properties of an endo-1,4-beta-glucanase from Clostridium josui (1989), J. Bacteriol., 171, 4076-4079.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Cysteine hydrochloride | slight activation | Ruminiclostridium josui |  |
| dithiothreitol | slight activation | Ruminiclostridium josui | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 1 mM, slight inhibition | Ruminiclostridium josui | |
| Cu2+ | CuCl2 | Ruminiclostridium josui | |
| glutathione | slight | Ruminiclostridium josui | |
| Hg2+ | HgCl2 | Ruminiclostridium josui | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 6.7 | - |
cellopentaose | - |
Ruminiclostridium josui | |
| 15.4 | - |
cellotetraose | - |
Ruminiclostridium josui | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| KCl | slight activation | Ruminiclostridium josui | |
| ZnCl2 | slight activation | Ruminiclostridium josui | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 45000 | - |
x * 45000, SDS-PAGE | Ruminiclostridium josui |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Ruminiclostridium josui | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Ruminiclostridium josui |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| additional information | stationary phase supernatant | Ruminiclostridium josui | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Ruminiclostridium josui |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| avicel + H2O | - |
Ruminiclostridium josui | ? | - |
? | |
| carboxymethylcellulose + H2O | - |
Ruminiclostridium josui | additional information | - |
? | |
| cellohexaose + H2O | - |
Ruminiclostridium josui | additional information | cellotriose + cellobiose + cellotetraose | ? | |
| cellopentaose + H2O | - |
Ruminiclostridium josui | additional information | cellobiose + cellotriose | ? | |
| cellotetraose + H2O | - |
Ruminiclostridium josui | 2 cellobiose | - |
? | |
| cellotriose + H2O | no activity | Ruminiclostridium josui | D-glucose + cellobiose | - |
? | |
| cellotriose + H2O | no reaction | Ruminiclostridium josui | D-glucose + cellobiose | - |
? | |
| additional information | no activity with cellobiose | Ruminiclostridium josui | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 45000, SDS-PAGE | Ruminiclostridium josui |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
hydrolysis of carboxymethylcellulose | Ruminiclostridium josui |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
1 h, more than 80% of the initial activity is retained | Ruminiclostridium josui |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | - |
hydrolysis of carboxymethylcellulose | Ruminiclostridium josui |
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