Any feedback?
Literature summary for 3.2.1.4 extracted from
- Cellulases from Thermoascus aurantiacus (1988), Methods Enzymol., 160, 300-307.
No PubMed abstract available
General Stability
| General Stability | Organism |
|---|---|
| dilute solutions, at 4°C, stable for several days | Thermoascus aurantiacus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Thermoascus aurantiacus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 34000 | - |
cellulase III, gel filtration | Thermoascus aurantiacus |
| 48000 | - |
cellulase II, gel filtration | Thermoascus aurantiacus |
| 78000 | - |
cellulase I, gel filtration | Thermoascus aurantiacus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermoascus aurantiacus | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | cellulase I contains 5.5% carbohydrate, cellulase II contains 2.6% carbohydrate, cellulase III contains 1.8% carbohydrate | Thermoascus aurantiacus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| cellulase I, II and III | Thermoascus aurantiacus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Thermoascus aurantiacus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, stable for at least 1 year | Thermoascus aurantiacus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| carboxymethylcellulose + H2O | hydrolyzed by cellulase I and III, no hydrolysis with cellulase II | Thermoascus aurantiacus | additional information | - |
? |  |
| carboxymethylpachyman + H2O | cellulase I | Thermoascus aurantiacus | ? | - |
? | |
| cellulose + H2O | partial degradation of native cellulose | Thermoascus aurantiacus | ? | - |
? | |
| glucan + H2O | yeast glucan | Thermoascus aurantiacus | ? | - |
? | |
| laminarin + H2O | cellulase I | Thermoascus aurantiacus | ? | - |
? | |
| lichenan + H2O | cellulase I, II and III | Thermoascus aurantiacus | ? | - |
? | |
| additional information | hydrolysis of filter paper | Thermoascus aurantiacus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 34000, cellulase III, SDS-PAGE | Thermoascus aurantiacus |
| monomer | 1 * 48000, cellulase II, SDS-PAGE | Thermoascus aurantiacus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
hydrolysis of yeast glucan with cellulase I or hydrolysis of carboxymethylcellulose with cellulase III | Thermoascus aurantiacus |
| 75 | - |
hydrolysis of carboxymethylcellulose with cellulase I | Thermoascus aurantiacus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
stable up to | Thermoascus aurantiacus |
| 75 | - |
less than 10% of the cellulolytic ativities remain after 1 h | Thermoascus aurantiacus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 5 | cellulase I, II and III | Thermoascus aurantiacus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 2 | 12 | cellulase II is stable | Thermoascus aurantiacus |
| 5 | 9 | cellulase I and III are stable | Thermoascus aurantiacus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
