Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | 40-50% inhibition at 2 mM | Anadara broughtonii | |
Cu2+ | 40-50% inhibition at 2 mM | Anadara broughtonii | |
Zn2+ | 40-50% inhibition at 2 mM | Anadara broughtonii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | activates 27% at 2 mM | Anadara broughtonii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
laminarin + H2O | Anadara broughtonii | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Anadara broughtonii | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | the enzyme comprises 23.65% carbohydrate content with O-linked glycan, no cleavage by PNGase F | Anadara broughtonii |
Purification (Comment) | Organism |
---|---|
native enzyme from mantle by ammonium sulfate fractionation, dialysis, anion exchange chromatography, hydrophobic interaction chromatography, gel filtration, and dialysis, to homogeneity | Anadara broughtonii |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mantle | - |
Anadara broughtonii | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
90.01 | - |
purified native enzyme, substrate laminarin, pH 6.0, 40°C | Anadara broughtonii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
barley beta-glucan + H2O | 40.18% activity compared to laminarin | Anadara broughtonii | ? | - |
? | |
laminarin + H2O | - |
Anadara broughtonii | ? | - |
? | |
laminarin + H2O | best substrate, the enzyme shows an endo-type cleavage mode and hydrolyzes laminarin into glucoses, disaccharides, trioligosaccharides, and tetraoligosaccharides | Anadara broughtonii | ? | - |
? | |
additional information | the enzyme exerts relatively high specific activity toward the glucans comprising beta-1,3-glycosidic bond, such as laminarin, pachyman, and barley beta-glucan, with relative activity of 100%, 78.86%, and 40.18%, respectively. Low activity with zymosan A, carboxymethylcellulose-Na, and agarose | Anadara broughtonii | ? | - |
? | |
pachyman + H2O | 78.86% ctivity compared to laminarin | Anadara broughtonii | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 51030, mass spectrometry, x * 40890, sequence calculation | Anadara broughtonii |
More | secondary structure analysis: alpha-helix comprising 55.6% of the structure, beta-sheet 22.4%, and random coil structures 22.0%. The total content of alpha-helix and beta-sheet accounts for more than 75.0% of the secondary structure, amino acid sequence comparisons | Anadara broughtonii |
Synonyms | Comment | Organism |
---|---|---|
beta-1,3-glucanase | - |
Anadara broughtonii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Anadara broughtonii |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 55 | 50% of maximal activity at 30°C and 50°C, maximal activity at 40°C, no activity at 55°C, profile overview | Anadara broughtonii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
purified native enzyme, 30 min, completely stable | Anadara broughtonii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Anadara broughtonii |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 9 | activity range, profile overview | Anadara broughtonii |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme exhibits immune stimulating effects via significantly increasing the phagocytic activity of macrophages and inducing the release of nitric oxide, tumor necrosis factor alpha, and interleukin-6 in RAW264.7 cells, overview | Anadara broughtonii |