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Literature summary for 3.2.1.39 extracted from

  • Li, C.; Wen, Y.; He, Y.; Zhu, J.; Yin, X.; Yang, J.; Zhang, L.; Song, L.; Xia, X.; Yu, R.
    Purification and characterization of a novel beta-1,3-glucanase from Arca inflata and its immune-enhancing effects (2019), Food Chem., 290, 1-9 .
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Ba2+ 40-50% inhibition at 2 mM Anadara broughtonii
Cu2+ 40-50% inhibition at 2 mM Anadara broughtonii
Zn2+ 40-50% inhibition at 2 mM Anadara broughtonii

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ activates 27% at 2 mM Anadara broughtonii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
laminarin + H2O Anadara broughtonii
-
?
-
?

Organism

Organism UniProt Comment Textmining
Anadara broughtonii
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein the enzyme comprises 23.65% carbohydrate content with O-linked glycan, no cleavage by PNGase F Anadara broughtonii

Purification (Commentary)

Purification (Comment) Organism
native enzyme from mantle by ammonium sulfate fractionation, dialysis, anion exchange chromatography, hydrophobic interaction chromatography, gel filtration, and dialysis, to homogeneity Anadara broughtonii

Source Tissue

Source Tissue Comment Organism Textmining
mantle
-
Anadara broughtonii
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
90.01
-
purified native enzyme, substrate laminarin, pH 6.0, 40°C Anadara broughtonii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
barley beta-glucan + H2O 40.18% activity compared to laminarin Anadara broughtonii ?
-
?
laminarin + H2O
-
Anadara broughtonii ?
-
?
laminarin + H2O best substrate, the enzyme shows an endo-type cleavage mode and hydrolyzes laminarin into glucoses, disaccharides, trioligosaccharides, and tetraoligosaccharides Anadara broughtonii ?
-
?
additional information the enzyme exerts relatively high specific activity toward the glucans comprising beta-1,3-glycosidic bond, such as laminarin, pachyman, and barley beta-glucan, with relative activity of 100%, 78.86%, and 40.18%, respectively. Low activity with zymosan A, carboxymethylcellulose-Na, and agarose Anadara broughtonii ?
-
?
pachyman + H2O 78.86% ctivity compared to laminarin Anadara broughtonii ?
-
?

Subunits

Subunits Comment Organism
? x * 51030, mass spectrometry, x * 40890, sequence calculation Anadara broughtonii
More secondary structure analysis: alpha-helix comprising 55.6% of the structure, beta-sheet 22.4%, and random coil structures 22.0%. The total content of alpha-helix and beta-sheet accounts for more than 75.0% of the secondary structure, amino acid sequence comparisons Anadara broughtonii

Synonyms

Synonyms Comment Organism
beta-1,3-glucanase
-
Anadara broughtonii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
Anadara broughtonii

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 55 50% of maximal activity at 30°C and 50°C, maximal activity at 40°C, no activity at 55°C, profile overview Anadara broughtonii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
purified native enzyme, 30 min, completely stable Anadara broughtonii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
Anadara broughtonii

pH Range

pH Minimum pH Maximum Comment Organism
3 9 activity range, profile overview Anadara broughtonii

General Information

General Information Comment Organism
physiological function the enzyme exhibits immune stimulating effects via significantly increasing the phagocytic activity of macrophages and inducing the release of nitric oxide, tumor necrosis factor alpha, and interleukin-6 in RAW264.7 cells, overview Anadara broughtonii