Literature summary for 3.2.1.39 extracted from

Niu, C.; Liu, C.; Li, Y.; Zheng, F.; Wang, J.; Li, Q.
Production of a thermostable 1,3-1,4-beta-glucanase mutant in Bacillus subtilis WB600 at a high fermentation capacity and its potential application in the brewing industry (2018), Int. J. Biol. Macromol., 107, 28-34 .

Search for more literature for 3.2.1.39

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Bacillus subtilis WB600 cells	Bacillus tequilensis

Protein Variants

Protein Variants	Comment	Organism
K20S/N31C/S40E/S43E/E46P/P102C/K117S/N125C/K165S/T187C/H205P	the mutant shows increased catalytic activity and stability compared to the wild type enzyme	Bacillus tequilensis

Inhibitors

Inhibitors	Comment	Organism	Structure
2-mercaptoethanol	33.7% residual activity at 10 mM	Bacillus tequilensis
Ca2+	86.7% residual activity at 10 mM	Bacillus tequilensis
Co2+	80.8% residual activity at 1 mM	Bacillus tequilensis
dithiothreitol	29.6% residual activity at 10 mM	Bacillus tequilensis
EDTA	13.2% residual activity at 10 mM	Bacillus tequilensis
Fe2+	5.9% residual activity at 10 mM	Bacillus tequilensis
Fe3+	10.1% residual activity at 10 mM	Bacillus tequilensis
Li+	51.3% residual activity at 10 mM	Bacillus tequilensis
Mg2+	61.3% residual activity at 10 mM	Bacillus tequilensis
Mn2+	68.1% residual activity at 10 mM	Bacillus tequilensis
additional information	not influenced by K+, Na+, or NH4+	Bacillus tequilensis
Zn2+	41.7% residual activity at 10 mM	Bacillus tequilensis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.272	-	Barley beta-glucan	wild type enzyme, at pH 6.5 and 40°C	Bacillus tequilensis

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	193.9% activity at 1 mM	Bacillus tequilensis
Mn2+	121.6% activity at 1 mM	Bacillus tequilensis

Organism

Organism	UniProt	Comment	Textmining
Bacillus tequilensis	A0A1W6AKT2	-	-
Bacillus tequilensis CGX 5-2	A0A1W6AKT2	-	-

Purification (Commentary)

Purification (Comment)	Organism
Q-Sepharose column chromatography	Bacillus tequilensis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
barley beta-glucan + H2O	-	Bacillus tequilensis	?	-	?
barley beta-glucan + H2O	-	Bacillus tequilensis CGX 5-2	?	-	?

Subunits

Subunits	Comment	Organism
?	x * 24000, SDS-PAGE	Bacillus tequilensis

Synonyms

Synonyms	Comment	Organism
Bgl	-	Bacillus tequilensis
endo-beta-1,3-glucanase	-	Bacillus tequilensis

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
70	-	-	Bacillus tequilensis

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
35	55	more than 70% activity between 35 and 55°C	Bacillus tequilensis

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
60	70	half-life values of the enzyme at 60°C and 70°C are 153.2 min and 99.6 min, respectively	Bacillus tequilensis

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
188.9	-	Barley beta-glucan	wild type enzyme, at pH 6.5 and 40°C	Bacillus tequilensis

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6	-	-	Bacillus tequilensis

pH Stability

pH Stability	pH Stability Maximum	Comment	Organism
4.5	5.5	there are 78.1% and 89.7% activities left after 1 h incubation at pH 4.5 and pH 5.5, respectively	Bacillus tequilensis

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
694.5	-	Barley beta-glucan	wild type enzyme, at pH 6.5 and 40°C	Bacillus tequilensis

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Release 2023.1 (January 2023)