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Literature summary for 3.2.1.39 extracted from

  • Niu, C.; Zhu, L.; Hill, A.; Alex Speers, R.; Li, Q.
    Construction of a highly thermostable 1,3-1,4-beta-glucanase by combinational mutagenesis and its potential application in the brewing industry (2017), Biotechnol. Lett., 39, 113-122 .
    View publication on PubMed
Search for more literature for 3.2.1.39

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Bacillus tequilensis

Protein Variants

Protein Variants Comment Organism
K20S/N31C/S40E/S43E/E46P/P102C/K117S/N125C/K165S/T187C/H205P the mutant shows 63% increased catalytic activity and stability compared to the wild type enzyme. Half-life values at 60°C and 70°C are 152 and 99 min, respectively Bacillus tequilensis

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol 41% residual activity at 10 mM Bacillus tequilensis
Ca2+ 84% residual activity at 10 mM Bacillus tequilensis
Co2+ 94% residual activity at 10 mM Bacillus tequilensis
Cu2+ 86% residual activity at 10 mM Bacillus tequilensis
dithiothreitol 35% residual activity at 10 mM Bacillus tequilensis
EDTA 31% residual activity at 10 mM Bacillus tequilensis
Fe2+ 8.4% residual activity at 10 mM Bacillus tequilensis
Fe3+ 6.5% residual activity at 10 mM Bacillus tequilensis
Li+ 51% residual activity at 10 mM Bacillus tequilensis
Mg2+ 50% residual activity at 10 mM Bacillus tequilensis
Mn2+ 55% residual activity at 10 mM Bacillus tequilensis
Zn2+ 35% residual activity at 10 mM Bacillus tequilensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.27
-
 Barley beta-glucan mutant enzyme K20S/N31C/S40E/S43E/E46P/P102C/K117S/N125C/K165S/T187C/H205P, at pH 6.5 and 40°C Bacillus tequilensis
0.3
-
 Barley beta-glucan wild type enzyme, at pH 6.5 and 40°C Bacillus tequilensis

Metals/Ions

Metals/Ions Comment Organism Structure
additional information not influenced by K+, Na+, or NH4+ Bacillus tequilensis

Organism

Organism UniProt Comment Textmining
Bacillus tequilensis
-
-
-
Bacillus tequilensis CGX 5-2
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Bacillus tequilensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
barley beta-glucan + H2O
-
 Bacillus tequilensis ?
-
 ?
barley beta-glucan + H2O
-
 Bacillus tequilensis CGX 5-2 ?
-
 ?

Synonyms

Synonyms Comment Organism
Bgl
-
 Bacillus tequilensis
endo-beta-1,3-glucanase
-
 Bacillus tequilensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Bacillus tequilensis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
35 55 more than 70% activity between 35 and 55°C Bacillus tequilensis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60 70 half-life values at 60°C and 70°C are 32.5 and 11 min, respectively. The enzyme is almost fully inactivated after incubation at 60°C for 140 min Bacillus tequilensis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
137
-
 Barley beta-glucan wild type enzyme, at pH 6.5 and 40°C Bacillus tequilensis
189
-
 Barley beta-glucan mutant enzyme K20S/N31C/S40E/S43E/E46P/P102C/K117S/N125C/K165S/T187C/H205P, at pH 6.5 and 40°C Bacillus tequilensis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
462
-
 Barley beta-glucan wild type enzyme, at pH 6.5 and 40°C Bacillus tequilensis
691
-
 Barley beta-glucan mutant enzyme K20S/N31C/S40E/S43E/E46P/P102C/K117S/N125C/K165S/T187C/H205P, at pH 6.5 and 40°C Bacillus tequilensis