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Literature summary for 3.2.1.39 extracted from

  • Kobayashi, T.; Uchimura, K.; Kubota, T.; Nunoura, T.; Deguchi, S.
    Biochemical and genetic characterization of beta-1,3 glucanase from a deep subseafloor Laceyella putida (2016), Appl. Microbiol. Biotechnol., 100, 203-214 .
    View publication on PubMed
Search for more literature for 3.2.1.39

Inhibitors

Inhibitors Comment Organism Structure
2-Hydroxy-5-nitrobenzylbromide 47% inhibition at 5 mM Laceyella putida
Hg2+ 39% inhibition at 1 mM Laceyella putida
monoiodoacetate 34% inhibition at 5 mM Laceyella putida
Pd2+ 23% inhibition at 1 mM Laceyella putida

Organism

Organism UniProt Comment Textmining
Laceyella putida A0A0P0UPC6
-
-
Laceyella putida JAM FM3001 A0A0P0UPC6
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE-Toyopearl column chromatography, butyl-Toyopearl column chromatography, and phenyl-Toyopearl column chromatography Laceyella putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
curdlan + H2O 100% activity Laceyella putida laminaribiose + laminaritriose
-
 ?
curdlan + H2O 100% activity Laceyella putida JAM FM3001 laminaribiose + laminaritriose
-
 ?
laminarin + H2O 47.6% activity compared to curdlan Laceyella putida ?
-
 ?
laminarin + H2O 47.6% activity compared to curdlan Laceyella putida JAM FM3001 ?
-
 ?
additional information no activity with xylan, colloidal chitin, carboxymethylcellulose, polygalacturonic acid, and pectin Laceyella putida ?
-
 ?
additional information no activity with xylan, colloidal chitin, carboxymethylcellulose, polygalacturonic acid, and pectin Laceyella putida JAM FM3001 ?
-
 ?
pachyman + H2O most favored substrate, 223% activity compared to curdlan Laceyella putida ?
-
 ?
pachyman + H2O most favored substrate, 223% activity compared to curdlan Laceyella putida JAM FM3001 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 36000, SDS-PAGE Laceyella putida
? x * 39836, calculated from amino acid sequence Laceyella putida

Synonyms

Synonyms Comment Organism
beta-1,3 glucanase
-
 Laceyella putida
GluA
-
 Laceyella putida

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
80
-
-
 Laceyella putida

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 80 the enzyme activity is drastically elevated above 40°C and reaches the maximum level at 80°C in 100 mM glycine-HCl buffer (pH 4.2) Laceyella putida

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30 75 the enzyme is stable during 30 min incubation from 30 to 75°C, where the maximal residual activity is 35 to 45% of the original activity Laceyella putida

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2
-
-
 Laceyella putida

pH Range

pH Minimum pH Maximum Comment Organism
3 7 the enzyme shows more than 60% activity between pH 3.0 and 7.0 and does not work well at pH 8.0 or above Laceyella putida

pH Stability

pH Stability pH Stability Maximum Comment Organism
2.4 8.7 when the enzyme is incubated at 40°C for 30 min, the enzyme is stable over the pH range of 2.4 to 8.7 Laceyella putida

pI Value

Organism Comment pI Value Maximum pI Value
Laceyella putida isoelectric focusing
-
 5

Expression

Organism Comment Expression
Laceyella putida enzyme expression is induced by N-acetylglucosamine up