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Literature summary for 3.2.1.39 extracted from

  • Torres, M.; Palomares, O.; Quiralte, J.; Pauli, G.; Rodriguez, R.; Villalba, M.
    An enzymatically active beta-1,3-glucanase from ash pollen with allergenic properties: a particular member in the Oleaceae family (2015), PLoS ONE, 10, e0133066.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.39

Application

Application Comment Organism
medicine Fra e 9 is a allergenic beta-1,3-glucanase from ash pollen. Both the N- and C-terminal domains retain comparable IgE-binding capacity when assayed with allergic sera Fraxinus excelsior

Cloned(Commentary)

Cloned (Comment) Organism
expression of the N- and C-terminal domains as independent recombinant proteins in Escherichia coli and Pichia pastoris Fraxinus excelsior

Protein Variants

Protein Variants Comment Organism
additional information the recombinant N-terminal domain has high hydrolytic activity on laminarin. The C-terminal domain of Fra e 9, a cysteine-rich compact structure, is able to bind laminarin Fraxinus excelsior

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
-
 Fraxinus excelsior
47760
-
-
 Fraxinus excelsior

Organism

Organism UniProt Comment Textmining
Fraxinus excelsior W8NXM8
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein the sequence includes two potential N-glycosylation consensus sites (NXS/T), N156 at the N-terminal domain, and N454 at the C-terminal domain Fraxinus excelsior
proteolytic modification the sequence includes a predicted cleavge site for processing after residue S29 Fraxinus excelsior

Source Tissue

Source Tissue Comment Organism Textmining
pollen
-
 Fraxinus excelsior
-

Subunits

Subunits Comment Organism
? x * 47760, calculated, x * 45000, SDS-PAGE Fraxinus excelsior

Synonyms

Synonyms Comment Organism
Fra e 9.01 allergen
-
 Fraxinus excelsior

pI Value

Organism Comment pI Value Maximum pI Value
Fraxinus excelsior calculated
-
 8.7