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Literature summary for 3.2.1.39 extracted from

  • Przybysz, A.; Volmer, A.A.; Westphal, A.H.; van Berkel, W.J.
    Bifunctional immobilization of a hyperthermostable endo-beta-1,3-glucanase (2014), Appl. Microbiol. Biotechnol., 98, 1155-1163.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pyrococcus furiosus

Protein Variants

Protein Variants Comment Organism
S296C the immobilized S296C variant shows extreme pH stability and can be repeatedly used at 60°C without significant activity loss of activity Pyrococcus furiosus

General Stability

General Stability Organism
operational stability of the immobilized S296C variant is optimal with milled beads suggesting applicability of the immobilized enzyme on the smallest particles for continuous substrate conversion under industrially relevant conditions. S296C bound to the large beads shows a somewhat lower operational stability Pyrococcus furiosus

Inhibitors

Inhibitors Comment Organism Structure
Laminarin
-
Pyrococcus furiosus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.2
-
Laminarin pH 6.5, 90°C, soluble S296C variant Pyrococcus furiosus
0.77
-
Laminarin pH 6.5, 90°C, S296C variant immobilized on beads of 0.02-0.04 mM size Pyrococcus furiosus
1
-
Laminarin pH 6.5, 90°C, S296C variant immobilized on beads of 0.063-0.09 mM size Pyrococcus furiosus
2.9
-
Laminarin pH 6.5, 90°C, S296C variant immobilized on beads of 0.15-0.3 mM size Pyrococcus furiosus

Organism

Organism UniProt Comment Textmining
Pyrococcus furiosus O73951
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Pyrococcus furiosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
laminarin + H2O
-
Pyrococcus furiosus ?
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
90
-
assay at Pyrococcus furiosus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
bifunctional immobilized S296C enzyme shows high operational stability and pH stability at 60°C Pyrococcus furiosus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
assay at Pyrococcus furiosus

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 6 60°C, 48 h, incubation does not significantly reduce the activity of the immobilized S296C variant Pyrococcus furiosus
10
-
60°C, 48 h, incubation does not significantly reduce the activity of the immobilized S296C variant Pyrococcus furiosus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.5
-
Laminarin pH 6.5, 90°C, S296C variant immobilized on beads of 0.02-0.04 mM size Pyrococcus furiosus
6.5
-
Laminarin pH 6.5, 90°C, soluble S296C variant Pyrococcus furiosus
22.7
-
Laminarin pH 6.5, 90°C, S296C variant immobilized on beads of 0.063-0.09 mM size Pyrococcus furiosus