Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pyrococcus furiosus |
Protein Variants | Comment | Organism |
---|---|---|
S296C | the immobilized S296C variant shows extreme pH stability and can be repeatedly used at 60°C without significant activity loss of activity | Pyrococcus furiosus |
General Stability | Organism |
---|---|
operational stability of the immobilized S296C variant is optimal with milled beads suggesting applicability of the immobilized enzyme on the smallest particles for continuous substrate conversion under industrially relevant conditions. S296C bound to the large beads shows a somewhat lower operational stability | Pyrococcus furiosus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Laminarin | - |
Pyrococcus furiosus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2 | - |
Laminarin | pH 6.5, 90°C, soluble S296C variant | Pyrococcus furiosus | |
0.77 | - |
Laminarin | pH 6.5, 90°C, S296C variant immobilized on beads of 0.02-0.04 mM size | Pyrococcus furiosus | |
1 | - |
Laminarin | pH 6.5, 90°C, S296C variant immobilized on beads of 0.063-0.09 mM size | Pyrococcus furiosus | |
2.9 | - |
Laminarin | pH 6.5, 90°C, S296C variant immobilized on beads of 0.15-0.3 mM size | Pyrococcus furiosus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus furiosus | O73951 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Pyrococcus furiosus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
laminarin + H2O | - |
Pyrococcus furiosus | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
assay at | Pyrococcus furiosus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
bifunctional immobilized S296C enzyme shows high operational stability and pH stability at 60°C | Pyrococcus furiosus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
assay at | Pyrococcus furiosus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | 6 | 60°C, 48 h, incubation does not significantly reduce the activity of the immobilized S296C variant | Pyrococcus furiosus |
10 | - |
60°C, 48 h, incubation does not significantly reduce the activity of the immobilized S296C variant | Pyrococcus furiosus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
3.5 | - |
Laminarin | pH 6.5, 90°C, S296C variant immobilized on beads of 0.02-0.04 mM size | Pyrococcus furiosus | |
6.5 | - |
Laminarin | pH 6.5, 90°C, soluble S296C variant | Pyrococcus furiosus | |
22.7 | - |
Laminarin | pH 6.5, 90°C, S296C variant immobilized on beads of 0.063-0.09 mM size | Pyrococcus furiosus |