Cloned (Comment) | Organism |
---|---|
expression in inactive form in Escherichia coli, expression as active enzyme in Pichia pastoris | Spodoptera frugiperda |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40400 | - |
x * 40400, calculated | Spodoptera frugiperda |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Spodoptera frugiperda | A6YQV6 | member of glycosyl hysrolase family 16 | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | sequence has five putative glycosylation sites at residues T33, S77, S324, E188 and E193 | Spodoptera frugiperda |
Purification (Comment) | Organism |
---|---|
from larval midgut | Spodoptera frugiperda |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
larva | - |
Spodoptera frugiperda | - |
midgut | mRNA coding for SLam is expressed along the whole midgut tissue | Spodoptera frugiperda | - |
additional information | mRNA coding for SLam is expressed along the whole midgut tissue, but not in the Malpighian tubules, fat body and carcass | Spodoptera frugiperda | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
4480 | - |
pH 9.0, 30°C | Spodoptera frugiperda |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
laminarin + H2O | - |
Spodoptera frugiperda | ? | - |
? | |
additional information | Enzyme shows low processivity of 0.4. No substrate: yeast beta-1,3-1,6-glucan or other polysaccharides. enzyme is unable to lyse Saccharomyces cerevisiae cells | Spodoptera frugiperda | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 40400, calculated | Spodoptera frugiperda |
Synonyms | Comment | Organism |
---|---|---|
SLam | - |
Spodoptera frugiperda |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
29 | - |
Laminarin | pH 9.0, 30°C | Spodoptera frugiperda |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Spodoptera frugiperda | calculated | - |
6.1 |
Organism | Comment | Expression |
---|---|---|
Spodoptera frugiperda | mRNA coding for SLam is expressed along the whole midgut tissue, but not in the Malpighian tubules, fat body and carcass | additional information |