Crystallization (Comment) | Organism |
---|---|
small angle X-ray scattering data and homology modeling. Enzyme displays a multi-domain structural architecture of a V-shape envelope with a catalytic domain flanked by two carbohydrate-binding modules | Thermotoga petrophila |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Km value for laminarin is 3.3 mg/ml | Thermotoga petrophila |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermotoga petrophila | A5IL44 | member of glycosyl hydrolase family 16 | - |
Thermotoga petrophila RKU-1 | A5IL44 | member of glycosyl hydrolase family 16 | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
21.4 | - |
substrate lichenan, pH 6.0, 91°C | Thermotoga petrophila |
41.55 | - |
substrate beta-glucan, pH 6.0, 91°C | Thermotoga petrophila |
48.1 | - |
substrate laminarin, pH 6.0, 91°C | Thermotoga petrophila |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-D-glucan + H2O | - |
Thermotoga petrophila | ? | - |
? | |
beta-D-glucan + H2O | - |
Thermotoga petrophila RKU-1 | ? | - |
? | |
laminarin + H2O | - |
Thermotoga petrophila | D-glucose + laminaribiose + laminaritriose | major products, enzyme most frequently attacks the bond between the 3rd and 4th residue from the non-reducing end | ? | |
laminarin + H2O | - |
Thermotoga petrophila RKU-1 | D-glucose + laminaribiose + laminaritriose | major products, enzyme most frequently attacks the bond between the 3rd and 4th residue from the non-reducing end | ? | |
lichenan + H2O | - |
Thermotoga petrophila | ? | - |
? | |
lichenan + H2O | - |
Thermotoga petrophila RKU-1 | ? | - |
? | |
additional information | specific cleavage of internal beta-1,3-bonds. No substrate: avicel, carboxymethyl cellulose and cellopentaose, xylan, arabinan and manan containing polysaccharides | Thermotoga petrophila | ? | - |
? | |
additional information | specific cleavage of internal beta-1,3-bonds. No substrate: avicel, carboxymethyl cellulose and cellopentaose, xylan, arabinan and manan containing polysaccharides | Thermotoga petrophila RKU-1 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
LAM | - |
Thermotoga petrophila |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
91 | - |
- |
Thermotoga petrophila |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
78 | - |
pH 6.0, 82% of maximum activity | Thermotoga petrophila |
95 | - |
pH 6.0, 86% of maximum activity | Thermotoga petrophila |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
stable | Thermotoga petrophila |
80 | - |
16 h, 60% residual activity | Thermotoga petrophila |
90 | - |
half-life 577 min | Thermotoga petrophila |
95 | - |
half-life 126 min | Thermotoga petrophila |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
680.3 | - |
Laminarin | pH 6.0, 91°C | Thermotoga petrophila |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.2 | - |
- |
Thermotoga petrophila |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 7 | more than 80% of maximum activity | Thermotoga petrophila |