Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.39 extracted from

  • Cheng, Y.; Hsieh, F.; Meng, M.
    Functional analysis of conserved aromatic amino acids in the discoidin domain of Paenibacillus beta-1,3-glucanase (2009), Microb. Cell Fact., 8, 62.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.39

Cloned(Commentary)

Cloned (Comment) Organism
expression of the isolated wild-type and mutant LamA discoidin domains as soluble His-tagged proteins in Escherichia coli strain BL21(DE3) Paenibacillus sp. CCRC 17245

Protein Variants

Protein Variants Comment Organism
W1679A site-directed mutagenesis, the mutation in the F5/8C module or DS domain decreases the protein affinity to laminarin Paenibacillus sp. CCRC 17245
W1688A site-directed mutagenesis, the mutation in the F5/8C module or DS domain decreases the protein affinity to laminarin Paenibacillus sp. CCRC 17245
W1729A site-directed mutagenesis, mutation in the F5/8C module or DS domain, the mutant domain forms inclusion bodies upon expression in Escherichia coli Paenibacillus sp. CCRC 17245
Y1714A site-directed mutagenesis, the mutation in the F5/8C module or DS domain decreases the protein affinity to laminarin Paenibacillus sp. CCRC 17245
Y1768A site-directed mutagenesis, the mutation in the F5/8C module or DS domain decreases the protein affinity to laminarin Paenibacillus sp. CCRC 17245

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
laminarin + H2O Paenibacillus sp. CCRC 17245
-
 ?
-
 ?
laminarin + H2O Paenibacillus sp. CCRC 17245 BCRC
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Paenibacillus sp. CCRC 17245 Q000P7 gene lamA
-
Paenibacillus sp. CCRC 17245 BCRC Q000P7 gene lamA
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged wild-type and mutant LamA discoidin domains from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration Paenibacillus sp. CCRC 17245

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
avicel + H2O i.e. microcrystalline cellulose Paenibacillus sp. CCRC 17245 ?
-
 ?
avicel + H2O i.e. microcrystalline cellulose Paenibacillus sp. CCRC 17245 BCRC ?
-
 ?
chitin + H2O
-
 Paenibacillus sp. CCRC 17245 ?
-
 ?
chitin + H2O
-
 Paenibacillus sp. CCRC 17245 BCRC ?
-
 ?
laminarin + H2O
-
 Paenibacillus sp. CCRC 17245 ?
-
 ?
laminarin + H2O
-
 Paenibacillus sp. CCRC 17245 BCRC ?
-
 ?
lichenan + H2O
-
 Paenibacillus sp. CCRC 17245 ?
-
 ?
lichenan + H2O
-
 Paenibacillus sp. CCRC 17245 BCRC ?
-
 ?

Subunits

Subunits Comment Organism
More the beta-1,3-glucanase LamA is highly modular, containing a signal sequence, three repeats of the S-layer homologous module, a segment with unknown function, a catalytic module of glycoside hydrolase family 16, GH16, four repeats of CBM4 family, and a F5/8C module representing a discoidin domain, DS domain, from N- to C-terminus. The recombinant isolated purified His-tagged DS domain forms dimers Paenibacillus sp. CCRC 17245

Synonyms

Synonyms Comment Organism
beta-1,3-glucanase
-
 Paenibacillus sp. CCRC 17245
LamA
-
 Paenibacillus sp. CCRC 17245
More LamA contains a catalytic module of the glycoside hydrolase family 16, GH16 Paenibacillus sp. CCRC 17245

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Paenibacillus sp. CCRC 17245

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
 melting temperatures of wild-type and mutant DS domains between 52.3°C and 58.3°C Paenibacillus sp. CCRC 17245

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.8
-
 assay at Paenibacillus sp. CCRC 17245