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Literature summary for 3.2.1.39 extracted from

  • Fibriansah, G.; Masuda, S.; Koizumi, N.; Nakamura, S.; Kumasaka, T.
    The 1.3 A crystal structure of a novel endo-beta-1,3-glucanase of glycoside hydrolase family 16 from alkaliphilic Nocardiopsis sp. strain F96 (2007), Proteins Struct. Funct. Bioinform., 69, 683-690.
    View publication on PubMed
Search for more literature for 3.2.1.39

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strain B834(DE3) Nocardiopsis sp.

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapour diffusion method with 0.1 M Tris-HCl (pH 8.0), 1.5 M ammonium sulfate, 0.1 M sodium chloride, and 1% (v/v) ethanol Nocardiopsis sp.

Protein Variants

Protein Variants Comment Organism
W118A the mutant almost abolishes its activity Nocardiopsis sp.

Inhibitors

Inhibitors Comment Organism Structure
3,4-epoxybutyl-beta-D-cellobioside
-
 Nocardiopsis sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ one calcium ion is bound on the convex face of the protein, suggesting to play a role in stabilizing the protein structure Nocardiopsis sp.

Organism

Organism UniProt Comment Textmining
Nocardiopsis sp.
-
-
-
Nocardiopsis sp. F96
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Nocardiopsis sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
laminarin + H2O
-
 Nocardiopsis sp. D-glucose + ?
-
 ?
laminarin + H2O
-
 Nocardiopsis sp. F96 D-glucose + ?
-
 ?

Synonyms

Synonyms Comment Organism
endo-beta-1,3-glucanase
-
 Nocardiopsis sp.