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Literature summary for 3.2.1.39 extracted from

  • Chiaraluce, R.; Florio, R.; Angelaccio, S.; Gianese, G.; van Lieshout, J.F.; van der Oost, J.; Consalvi, V.
    Tertiary structure in 7.9 M guanidinium chloride--the role of Glu53 and Asp287 in Pyrococcus furiosus endo-beta-1,3-glucanase (2007), FEBS J., 274, 6167-6179.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strain BL21(DE3) Pyrococcus furiosus

Protein Variants

Protein Variants Comment Organism
D287A decreased activity Pyrococcus furiosus
E53A decreased activity Pyrococcus furiosus
E53A/D287A decreased activity Pyrococcus furiosus

Organism

Organism UniProt Comment Textmining
Pyrococcus furiosus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Pyrococcus furiosus

Renatured (Commentary)

Renatured (Comment) Organism
refolding is started by 20fold dilution of the unfolding mixture, at 20°C, into solutions of buffer containing decreasing guanidinium chloride concentrations Pyrococcus furiosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-glucan + H2O
-
Pyrococcus furiosus ?
-
?
laminarin + H2O
-
Pyrococcus furiosus D-glucose + ?
-
?

Synonyms

Synonyms Comment Organism
endo-beta-1,3-glucanase
-
Pyrococcus furiosus