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Literature summary for 3.2.1.39 extracted from

  • Salazar, O.; Molitor, J.; Lienqueo, M.E.; Asenjo, J.A.
    Overproduction, purification, and characterization of beta-1,3-glucanase type II in Escherichia coli (2001), Protein Expr. Purif., 23, 219-225.
    View publication on PubMed
Search for more literature for 3.2.1.39

Cloned(Commentary)

Cloned (Comment) Organism
-
 Cellulosimicrobium cellulans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.688
-
 Laminarin pH 8.0, 37°C Cellulosimicrobium cellulans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
 x * 43000, recombinant protein with his6-tag, SDS-PAGE Cellulosimicrobium cellulans

Organism

Organism UniProt Comment Textmining
Cellulosimicrobium cellulans
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme with His6-tag, expression in Escherichia coli Cellulosimicrobium cellulans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
8
-
 pH 8.0, 37°C Cellulosimicrobium cellulans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
laminarin + H2O
-
 Cellulosimicrobium cellulans ?
-
 ?

Subunits

Subunits Comment Organism
? x * 43000, recombinant protein with his6-tag, SDS-PAGE Cellulosimicrobium cellulans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
14.88
-
 Laminarin pH 8.0, 37°C Cellulosimicrobium cellulans

pI Value

Organism Comment pI Value Maximum pI Value
Cellulosimicrobium cellulans isoelectric focusing
-
 6