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Literature summary for 3.2.1.39 extracted from

  • El-Katatny, M.H.; Gudelj, M.; Robra, K.H.; Elnaghy, M.A.; Gubitz, G.M.
    Characterization of a chitinase and an endo-beta-1,3-glucanase from Trichoderma harzianum Rifai T24 involved in control of the phytopathogen Sclerotium rolfsii (2001), Appl. Microbiol. Biotechnol., 56, 137-143.
    View publication on PubMed

Application

Application Comment Organism
biotechnology enzyme inhibits growth of phytopathogen Scerotium rolfsii with 50’% effective dose value of 0.0027 mg/ml Trichoderma harzianum

Inhibitors

Inhibitors Comment Organism Structure
Hg2+ 5 mM, 93% inhibition Trichoderma harzianum
additional information not inhibitory: glucono-delta-lactone Trichoderma harzianum

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Trichoderma harzianum
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
74000
-
gel filtration Trichoderma harzianum

Organism

Organism UniProt Comment Textmining
Trichoderma harzianum
-
Rifai T24
-
Trichoderma harzianum Rifai T24
-
Rifai T24
-

Purification (Commentary)

Purification (Comment) Organism
-
Trichoderma harzianum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
laminarin + H2O
-
Trichoderma harzianum ?
-
?
laminarin + H2O
-
Trichoderma harzianum Rifai T24 ?
-
?
additional information specifically cleaves beta-1,3-linkages in polysaccharides Trichoderma harzianum ?
-
?
additional information specifically cleaves beta-1,3-linkages in polysaccharides Trichoderma harzianum Rifai T24 ?
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
Trichoderma harzianum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
half-life more than 24 h Trichoderma harzianum
50
-
half-life 38 min Trichoderma harzianum
60
-
half-life 20 min Trichoderma harzianum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
-
Trichoderma harzianum

pI Value

Organism Comment pI Value Maximum pI Value
Trichoderma harzianum isoelectric focusing
-
6