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Literature summary for 3.2.1.39 extracted from
- beta-1,3-Glucanase produced by alkalophilic bacteria Bacillus No. K-12-5 (1973), Agric. Biol. Chem., 37, 1449-1456.
No PubMed abstract available
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Bacillus sp. (in: Bacteria) | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40000 | - |
SDS-PAGE | Bacillus sp. (in: Bacteria) |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cell wall glucan + H2O | Bacillus sp. (in: Bacteria) | from fungi | reducing sugar + ? | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus sp. (in: Bacteria) | - |
alkalophilic strain K-12-5 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| to homogeneity, 3step chromatography | Bacillus sp. (in: Bacteria) |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture filtrate | - |
Bacillus sp. (in: Bacteria) | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 15 | - |
- |
Bacillus sp. (in: Bacteria) |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-O-beta-D-Glc-D-Glc-D-Glc-D-Glc + H2O | release of laminaritriose and glucose | Bacillus sp. (in: Bacteria) | ? | - |
? | |
| 3-O-beta-D-Glc-D-Glc-D-Glc-D-Glc + H2O | laminaritetraose | Bacillus sp. (in: Bacteria) | ? | - |
? | |
| cell wall glucan + H2O | from fungi | Bacillus sp. (in: Bacteria) | reducing sugar + ? | - |
? | |
| laminarin + H2O | specifically hydrolyzes (1-3)-beta-glycosidic linkages, predominant products are laminaribiose and laminaritriose, minor amounts of glucose and higher oligosaccharides | Bacillus sp. (in: Bacteria) | D-glucose + ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
- |
Bacillus sp. (in: Bacteria) |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 60 | - |
Bacillus sp. (in: Bacteria) |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 8 | - |
Bacillus sp. (in: Bacteria) |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
most stable | Bacillus sp. (in: Bacteria) |
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Release 2023.1 (January 2023)
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