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Literature summary for 3.2.1.39 extracted from
- Purification and characterization of an endo-beta-1,3-glucanase from Trichoderma harzianum (1996), Can. J. Microbiol., 42, 1039-1044.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | 21% inhibition at 2 mM | Trichoderma harzianum |  |
| Hg2+ | strong inhibition at 2 mM | Trichoderma harzianum | |
| SDS | 43% inhibition at 2 mM | Trichoderma harzianum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
Laminarin | value in mg/ml | Trichoderma harzianum | |
| additional information | - |
Laminarin | 1.18 | Trichoderma harzianum | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Trichoderma harzianum | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | slight activation at 2 mM | Trichoderma harzianum | |
| Zn2+ | slight activation at 2 mM | Trichoderma harzianum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 36000 | - |
SDS-PAGE | Trichoderma harzianum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cell wall glucan + H2O | Trichoderma harzianum | from different fungi, containing beta-1,3-, beta-1,4 and/or beta-1,6-linkages | reducing sugar + ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Trichoderma harzianum | - |
enzyme synthesis induced by growing on chitin-containing medium | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| to homogeneity, 2step chromatography | Trichoderma harzianum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture filtrate | - |
Trichoderma harzianum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 711 | - |
- |
Trichoderma harzianum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| beta-glucan + H2O | beta-1,3- and beta-1,4-linkages | Trichoderma harzianum | ? | - |
? | |
| cell wall glucan + H2O | from different fungi, containing beta-1,3-, beta-1,4 and/or beta-1,6-linkages | Trichoderma harzianum | reducing sugar + ? | - |
? | |
| laminarin + H2O | only active against glucans containing beta-1,3-linkages | Trichoderma harzianum | D-glucose + ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| endo-beta-1,3-glucanase | - |
Trichoderma harzianum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
- |
Trichoderma harzianum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
stable for 90 min | Trichoderma harzianum |
| 50 | - |
50% activity | Trichoderma harzianum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.4 | - |
- |
Trichoderma harzianum |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 2.6 | - |
60% remaining activity | Trichoderma harzianum |
| 6.2 | - |
60% remaining activity | Trichoderma harzianum |
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