Any feedback?
Literature summary for 3.2.1.39 extracted from
- Purification and characterization of a (1-3)-beta-D-glucan endohydrolase from rice (Oryza sativa) bran (1997), Carbohydr. Res., 297, 365-374.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.04 | - |
Laminarin | - |
Oryza sativa |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 29000 | - |
SDS-PAGE, gel filtration | Oryza sativa |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Oryza sativa | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| to homogeneity, 4step chromatography | Oryza sativa |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seed coat | - |
Oryza sativa | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 500 | - |
- |
Oryza sativa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (1-3:1-4)-beta-D-glucan + H2O | very low activity | Oryza sativa | ? | - |
? | |
| (1-3:1-4)-beta-D-glucan + H2O | lichenan | Oryza sativa | ? | - |
? | |
| curdlan + H2O | from Alcaligenes faecalis | Oryza sativa | ? | - |
? | |
| curdlan + H2O | insoluble reduced 1,3-beta-glucan | Oryza sativa | ? | - |
? | |
| laminarin + H2O | from Laminaria digitata, lower rates are from Laminaria hyperborea and Eisenia bicyclis, best substrates are unbranched (1-3)-beta-D-glucans | Oryza sativa | D-glucose + ? | - |
? | |
| O-carboxymethyl-(1,3:1,6)-beta-D-glucan + H2O | from yeast, low activity | Oryza sativa | ? | - |
? | |
| O-carboxymethyl-pachyman + H2O | unbranched (1-3)-beta-glucan from Poria cocos | Oryza sativa | ? | - |
? | |
| schizophyllan + H2O | from Schizophyllum commune, low activity | Oryza sativa | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 29000, SDS-PAGE, gel filtration | Oryza sativa |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| (1-3)-beta-D-glucan endohydrolase | - |
Oryza sativa |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Oryza sativa |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | 70 | activity rapidly lost | Oryza sativa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Oryza sativa |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3.5 | 6 | rapid inactivation below and above | Oryza sativa |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
