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Literature summary for 3.2.1.39 extracted from
- Production, purification, and properties of an endo-1,3-beta-glucanase from Basidiomycete Agaricus bisporus (1999), Curr. Microbiol., 38, 190-193.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Hg2+ | 52% inhibition for laminarin, 62% inhibition for pustulan | Agaricus bisporus |  |
| iodoacetamide | slight inhibition | Agaricus bisporus | |
| KMnO4 | 55% inhibition for laminarin, 85% inhibition for pustulan | Agaricus bisporus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
Laminarin | value in mg/ml | Agaricus bisporus | |
| additional information | - |
Laminarin | 0.35 | Agaricus bisporus | |
| additional information | - |
pustulan | value in mg/ml: 0.07 | Agaricus bisporus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Agaricus bisporus | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 32000 | 33700 | native PAGE, SDS-PAGE, MALDI-MS | Agaricus bisporus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Agaricus bisporus | - |
enzyme synthesis induced by culturing on media containing Agaricus bisporus fruiting body cell walls and fructose | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | - |
Agaricus bisporus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| to homogeneity, gel filtration, preparative PAGE | Agaricus bisporus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture filtrate | - |
Agaricus bisporus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 66.4 | - |
- |
Agaricus bisporus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| laminarin + H2O | best substrate, specific for for 1,3-beta-glucans in which 1,6-beta-linkages are present | Agaricus bisporus | D-glucose + ? | - |
? | |
| pustulan + H2O | beta-1,6-glucan, low activity | Agaricus bisporus | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
- |
Agaricus bisporus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | - |
- |
Agaricus bisporus |
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