Literature summary for 3.2.1.38 extracted from

Yoshida, S.; Park, D.S.; Bae, B.; Mackie, R.; Cann, I.K.; Nair, S.K.
Structural and functional analyses of a glycoside hydrolase family 5 enzyme with an unexpected beta-fucosidase activity (2011), Biochemistry, 50, 3369-3375.

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Prevotella bryantii

Crystallization (Commentary)

Crystallization (Comment)	Organism
to 2.2 A resolution, space group C222. The overall topology consists of a central catalytic (alpha/beta)8 barrel composed of residues Thr14 through Phe413 flanked by amino- and carboxy-terminal extensions that consist of sheets that form an independent beta-sandwich domain. This small beta domain associates with the backside of the catalytic domain, and two domains associate through hydrophobic interactions	Prevotella bryantii

Crystallization (Comment)

Organism

to 2.2 A resolution, space group C222. The overall topology consists of a central catalytic (alpha/beta)8 barrel composed of residues Thr14 through Phe413 flanked by amino- and carboxy-terminal extensions that consist of sheets that form an independent beta-sandwich domain. This small beta domain associates with the backside of the catalytic domain, and two domains associate through hydrophobic interactions

Prevotella bryantii

Protein Variants

Protein Variants	Comment	Organism
E205A	complete loss of activity	Prevotella bryantii
E239A	complete loss of activity	Prevotella bryantii

Organism

Organism	UniProt	Comment	Textmining
Prevotella bryantii	-	glycoside hydrolase family 5 enzyme	-
Prevotella bryantii B14	-	glycoside hydrolase family 5 enzyme	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
4-nitrophenyl alpha-L-arabinopyranoside + H2O	about 60% of the activity with 4-nitrophenyl beta-D-fucopyranoside	Prevotella bryantii	4-nitrophenol + alpha-L-arabinopyranose	-	?
4-nitrophenyl alpha-L-arabinopyranoside + H2O	about 60% of the activity with 4-nitrophenyl beta-D-fucopyranoside	Prevotella bryantii B14	4-nitrophenol + alpha-L-arabinopyranose	-	?
4-nitrophenyl beta-D-fucopyranoside + H2O	best substrate	Prevotella bryantii	4-nitrophenol + beta-D-fucopyranose	-	?
4-nitrophenyl beta-D-fucopyranoside + H2O	best substrate	Prevotella bryantii B14	4-nitrophenol + beta-D-fucopyranose	-	?
4-nitrophenyl beta-D-galactopyranoside + H2O	about 30% of the activity with 4-nitrophenyl beta-D-fucopyranoside	Prevotella bryantii	4-nitrophenol + beta-D-galactopyranose	-	?
4-nitrophenyl beta-D-galactopyranoside + H2O	about 30% of the activity with 4-nitrophenyl beta-D-fucopyranoside	Prevotella bryantii B14	4-nitrophenol + beta-D-galactopyranose	-	?
additional information	no substrate: xylan polysaccharides	Prevotella bryantii	?	-	?
additional information	no substrate: xylan polysaccharides	Prevotella bryantii B14	?	-	?

Synonyms

Synonyms	Comment	Organism
FucA	-	Prevotella bryantii
Pb280	-	Prevotella bryantii

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
5	-	protein precipitates below	Prevotella bryantii

pH Stability

pH Stability	pH Stability Maximum	Comment	Organism
5	-	protein precipitates below	Prevotella bryantii