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Literature summary for 3.2.1.38 extracted from

  • Nunoura, N.; Ohdan, K.; Yano, T.; Yamamoto, K.; Kumagai, H.
    Purification and characterization of beta-D-glucosidase (beta-D-fucosidase) from Bifidobacterium berve clb acclimated to cellobiose (1996), Biosci. Biotechnol. Biochem., 60, 188-193.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
beta-D-fructose promotes beta-D-fucosidase activity, not beta-D-glucosidase activity Bifidobacterium breve clb
beta-D-glucose promotes beta-D-fucosidase activity at 50 mM, not beta-D-glucosidase activity Bifidobacterium breve clb
beta-D-mannose promotes beta-D-fucosidase activity, not beta-D-glucosidase activity Bifidobacterium breve clb
beta-D-xylose promotes beta-D-fucosidase activity, not beta-D-glucosidase activity Bifidobacterium breve clb

Application

Application Comment Organism
synthesis synthesis of fucosylsugars using the transglycosylation activity Bifidobacterium breve clb

Inhibitors

Inhibitors Comment Organism Structure
1-amino-1-deoxy-D-glucose
-
Bifidobacterium breve clb
1-butanol inhibition above 50 mM Bifidobacterium breve clb
1-propanol inhibition above 50 mM Bifidobacterium breve clb
4-O-beta-D-galactopyranosyl-D-glucose
-
Bifidobacterium breve clb
4-O-beta-D-glucopyranosyl-D-glucose cellobiose Bifidobacterium breve clb
acetone inhibition at low concentrations Bifidobacterium breve clb
acetonitrile inhibition at low concentrations Bifidobacterium breve clb
Ag+ inhibition at 1 mM Bifidobacterium breve clb
beta-D-fructofuranosyl-alpha-D-glucopyranoside
-
Bifidobacterium breve clb
beta-D-fucose inhibition of beta-D-glucosidase activity, beta-D-fucosidase activity hardly affected Bifidobacterium breve clb
beta-D-glucose inhibition of beta-D-glucosidase activity, not beta-D-fucosidase activity Bifidobacterium breve clb
Cu2+ inhibition at 1 mM Bifidobacterium breve clb
ethanol inhibition above 50 mM Bifidobacterium breve clb
Hg2+ inhibition at 1 mM Bifidobacterium breve clb
methanol inhibition above 50 mM Bifidobacterium breve clb
p-chloromercuribenzoate inhibition at 0.01 mM Bifidobacterium breve clb

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.7
-
p-nitrophenyl-beta-D-fucoside
-
Bifidobacterium breve clb
1.3
-
p-nitrophenyl-beta-D-glucoside
-
Bifidobacterium breve clb

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
47000 48000 native gradient PAGE, SDS-PAGE Bifidobacterium breve clb

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4-nitrophenyl beta-D-fucoside + H2O Bifidobacterium breve clb 80% activity compared to beta-D-glucoside beta-D-fucose + 4-nitrophenol
-
?
4-nitrophenyl beta-D-glucoside + H2O Bifidobacterium breve clb 20% more activity than on beta-D-fucoside, addition of D-glucose leads to formation of fucosylglucose as a product of transglycosylation reaction beta-D-glucose + 4-nitrophenol
-
?

Organism

Organism UniProt Comment Textmining
Bifidobacterium breve clb
-
increased activity after acclimation with cellobiose
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity by chromatography steps Bifidobacterium breve clb

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
Bifidobacterium breve clb
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
54.6
-
for beta-D-fucosidase activity Bifidobacterium breve clb
67.3
-
for beta-D-glucosidase activity Bifidobacterium breve clb

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2-hydroxymethylphenyl)-beta-D-glucopyranoside + H2O
-
Bifidobacterium breve clb 2-hydroxymethylphenol + beta-D-glucose
-
?
2-O-beta-D-glucopyranosyl-D-glucopyranose + H2O sophorose Bifidobacterium breve clb beta-D-glucose + D-glucose
-
?
3-O-beta-D-glucopyranosyl-D-glucopyranose + H2O laminaribiose Bifidobacterium breve clb beta-D-glucose + D-glucose
-
?
4-methylumbelliferyl-beta-D-glucoside + H2O increased activity after acclimation of bacteria to cellobiose medium Bifidobacterium breve clb 4-methylumbelliferone + beta-D-glucose
-
?
4-nitrophenyl beta-D-fucoside + H2O 80% activity compared to beta-D-glucoside Bifidobacterium breve clb beta-D-fucose + 4-nitrophenol
-
?
4-nitrophenyl beta-D-glucoside + H2O 20% more activity than on beta-D-fucoside, addition of D-glucose leads to formation of fucosylglucose as a product of transglycosylation reaction Bifidobacterium breve clb beta-D-glucose + 4-nitrophenol
-
?
4-O-beta-D-glucopyranosyl-D-glucose + H2O cellobiose, weak activity after prolonged incubation Bifidobacterium breve clb beta-D-glucose + D-glucose
-
?
6-O-beta-D-glucopyranosyl-D-glucose + H2O gentiobiose Bifidobacterium breve clb beta-D-glucose + D-glucose
-
?
p-nitrophenyl-beta-D-fucoside + H2O 80% activity compared to the activity towards beta-D-glucoside Bifidobacterium breve clb p-nitrophenol + beta-D-fucose
-
?
p-nitrophenyl-beta-D-fucoside + H2O contains a single active site for hydrolysis of both p-nitrophenyl-beta-D-fucoside and p-nitrophenyl-beta-D-glucoside Bifidobacterium breve clb p-nitrophenol + beta-D-fucose
-
?
p-nitrophenyl-beta-D-galactoside + H2O low activity Bifidobacterium breve clb p-nitrophenol + beta-D-galactose
-
?
p-nitrophenyl-beta-D-glucoside + H2O
-
Bifidobacterium breve clb p-nitrophenol + beta-D-glucose
-
?
p-nitrophenyl-beta-D-xyloside + H2O low activity Bifidobacterium breve clb p-nitrophenol + beta-D-xylose
-
?

Subunits

Subunits Comment Organism
monomer 1 * 47000-48000, SDS-PAGE, native PAGE Bifidobacterium breve clb

Synonyms

Synonyms Comment Organism
beta-D-glucosidase/beta-D-fucosidase
-
Bifidobacterium breve clb

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
Bifidobacterium breve clb

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
stable up to, 60% activity after incubation at 50°C Bifidobacterium breve clb

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
Bifidobacterium breve clb

pH Range

pH Minimum pH Maximum Comment Organism
5 7 90% activity between pH 5-7, 70% activity remained at pH 4.5 and pH 8.5 Bifidobacterium breve clb