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Literature summary for 3.2.1.38 extracted from

  • Srisomsap, C.; Svasti, J.; Surarit, R.; Champattanachai, V.; Sawangareetrakul, P.; Boonpuan, K.; Subhasitanont, P.; Chokchaichamnankit, D.
    Isolation and characterization of an enzyme with beta-glucosidase and beta-fucosidase activities from Dalbergia cochinchinensis Pierre (1996), J. Biochem., 119, 585-590.
    View publication on PubMed
Search for more literature for 3.2.1.38

Inhibitors

Inhibitors Comment Organism Structure
glucono 1,5-lactone inhibitory between 0.1 and 1 mM Dalbergia cochinchinensis
Hg2+ strong inhibition at 0.001 mM Dalbergia cochinchinensis
p-chloromercuribenzoate strong inhibition between 0.01 mM and 0.001 mM Dalbergia cochinchinensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.54
-
 p-nitrophenyl-beta-D-fucoside
-
 Dalbergia cochinchinensis
5.37
-
 p-nitrophenyl-beta-D-glucoside
-
 Dalbergia cochinchinensis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
66000
-
 SDS-PAGE Dalbergia cochinchinensis

Organism

Organism UniProt Comment Textmining
Dalbergia cochinchinensis
-
 thai rosewood
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity by isoelectric focusing and chromatography steps Dalbergia cochinchinensis

Source Tissue

Source Tissue Comment Organism Textmining
seed highest level compared to other tissues Dalbergia cochinchinensis
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
48
-
 for beta-D-glucosidase activity Dalbergia cochinchinensis
62
-
 for beta-D-fucosidase activity Dalbergia cochinchinensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2-hydroxymethylphenyl)-beta-D-glucopyranoside + H2O salicin, low activity Dalbergia cochinchinensis 2-hydroxymethylphenol + beta-D-glucose
-
 ?
(R)-alpha-[(6-O-beta-D-glucopyranosyl-beta-D-glucopyranosyl)oxy]benzeneacetonitrile + H2O amygdalin, low activity Dalbergia cochinchinensis ?
-
 ?
p-nitrophenyl-alpha-L-arabinoside + H2O low activity Dalbergia cochinchinensis p-nitrophenol + alpha-L-arabinose
-
 ?
p-nitrophenyl-beta-D-fucoside + H2O high activity Dalbergia cochinchinensis p-nitrophenol + beta-D-fucose
-
 ?
p-nitrophenyl-beta-D-galactoside + H2O low activity Dalbergia cochinchinensis p-nitrophenol + beta-D-galactose
-
 ?
p-nitrophenyl-beta-D-glucoside + H2O high activity Dalbergia cochinchinensis p-nitrophenol + beta-D-glucose
-
 ?
p-nitrophenyl-beta-D-xyloside + H2O low activity Dalbergia cochinchinensis p-nitrophenol + beta-D-xylose
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Dalbergia cochinchinensis