Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ethanol | 15% v/v, slight activation when incubated up to 60 min | Anoxybacillus sp. 3M | |
methanol | 15% v/v, slight activation when incubated up to 60 min | Anoxybacillus sp. 3M | |
n-butanol | 15% v/v, slight activation when incubated up to 60 min | Anoxybacillus sp. 3M | |
SDS | activity is moderately stimulated. 10 mM, 33.6% increase inactivity | Anoxybacillus sp. 3M |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-propanol | - |
Anoxybacillus sp. 3M | |
Ag+ | 1 mM, 98% loss of activity | Anoxybacillus sp. 3M | |
Ca2+ | 1 mM, 12% loss of activity. 10 mM, 18% loss of activity | Anoxybacillus sp. 3M | |
Cu2+ | 1 mM, 38% loss of activity. 10 mM, 70% loss of activity | Anoxybacillus sp. 3M | |
D-xylose | - |
Anoxybacillus sp. 3M | |
Fe2+ | 1 mM, 6% loss of activity. 10 mM, 47% loss of activity | Anoxybacillus sp. 3M | |
Hg2+ | 1 mM, complete loss of activity | Anoxybacillus sp. 3M | |
Mn2+ | 1 mM, 10% loss of activity. 10 mM, 12% loss of activity | Anoxybacillus sp. 3M | |
Ni2+ | 1 mM, 9% loss of activity. 10 mM, 15% loss of activity | Anoxybacillus sp. 3M |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.19 | - |
4-nitrophenyl beta-D-xylopyranoside | pH and temperature not specified in the publication | Anoxybacillus sp. 3M |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
intracellular | - |
Anoxybacillus sp. 3M | 5622 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
140000 | - |
- |
Anoxybacillus sp. 3M |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Anoxybacillus sp. 3M | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Anoxybacillus sp. 3M |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl beta-D-xylopyranoside + H2O | preferred substrate of the purified enzyme | Anoxybacillus sp. 3M | 4-nitrophenol + beta-D-xylopyranose | - |
? | |
additional information | no detectable alpha-L-arabinofuranosidase, alpha- and beta-D-glucosidase activities are observed. The enzyme hydrolyzes xylooligomers into xylose and catalyzes transxylosilation reactions also in presence of alcohols as acceptors, producing xylo-oligosaccharides and alkyl-xylosides | Anoxybacillus sp. 3M | ? | - |
? | |
xylobiose + H2O | - |
Anoxybacillus sp. 3M | 2 beta-D-xylose | - |
? | |
xylotriose + H2O | - |
Anoxybacillus sp. 3M | beta-D-xylose + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | - |
Anoxybacillus sp. 3M |
Synonyms | Comment | Organism |
---|---|---|
AbXyl | - |
Anoxybacillus sp. 3M |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | 65 | - |
Anoxybacillus sp. 3M |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | 70 | 55°C: 83% of maximal activity, 70°C: 41% of maximal activity | Anoxybacillus sp. 3M |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
half-life: 10 h | Anoxybacillus sp. 3M |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
453.29 | - |
4-nitrophenyl beta-D-xylopyranoside | pH and temperature not specified in the publication | Anoxybacillus sp. 3M |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
- |
Anoxybacillus sp. 3M |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.5 | 7 | pH 4.5: 46% of maximal activity, pH 7.0: 50% of maximal activity | Anoxybacillus sp. 3M |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
24 h, 78% residual activity | Anoxybacillus sp. 3M |
8 | - |
24 h, 88% residual activity | Anoxybacillus sp. 3M |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
21.25 | - |
D-xylose | pH and temperature not specified in the publication | Anoxybacillus sp. 3M |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2322 | - |
4-nitrophenyl beta-D-xylopyranoside | pH and temperature not specified in the publication | Anoxybacillus sp. 3M |