Literature summary for 3.2.1.37 extracted from

Marcolongo, L.; La Cara, F.; Del Monaco, G.; Paixao, S.M.; Alves, L.; Marques, I.P.; Ionata, E.
A novel beta-xylosidase from Anoxybacillus sp. 3M towards an improved agro-industrial residues saccharification (2019), Int. J. Biol. Macromol., 122, 1224-1234 .

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Activating Compound

Activating Compound	Comment	Organism	Structure
ethanol	15% v/v, slight activation when incubated up to 60 min	Anoxybacillus sp. 3M
methanol	15% v/v, slight activation when incubated up to 60 min	Anoxybacillus sp. 3M
n-butanol	15% v/v, slight activation when incubated up to 60 min	Anoxybacillus sp. 3M
SDS	activity is moderately stimulated. 10 mM, 33.6% increase inactivity	Anoxybacillus sp. 3M

Inhibitors

Inhibitors	Comment	Organism	Structure
2-propanol	-	Anoxybacillus sp. 3M
Ag+	1 mM, 98% loss of activity	Anoxybacillus sp. 3M
Ca2+	1 mM, 12% loss of activity. 10 mM, 18% loss of activity	Anoxybacillus sp. 3M
Cu2+	1 mM, 38% loss of activity. 10 mM, 70% loss of activity	Anoxybacillus sp. 3M
D-xylose	-	Anoxybacillus sp. 3M
Fe2+	1 mM, 6% loss of activity. 10 mM, 47% loss of activity	Anoxybacillus sp. 3M
Hg2+	1 mM, complete loss of activity	Anoxybacillus sp. 3M
Mn2+	1 mM, 10% loss of activity. 10 mM, 12% loss of activity	Anoxybacillus sp. 3M
Ni2+	1 mM, 9% loss of activity. 10 mM, 15% loss of activity	Anoxybacillus sp. 3M

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.19	-	4-nitrophenyl beta-D-xylopyranoside	pH and temperature not specified in the publication	Anoxybacillus sp. 3M

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
intracellular	-	Anoxybacillus sp. 3M	5622	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
140000	-	-	Anoxybacillus sp. 3M

Organism

Organism	UniProt	Comment	Textmining
Anoxybacillus sp. 3M	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Anoxybacillus sp. 3M

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-nitrophenyl beta-D-xylopyranoside + H2O	preferred substrate of the purified enzyme	Anoxybacillus sp. 3M	4-nitrophenol + beta-D-xylopyranose	-	?
additional information	no detectable alpha-L-arabinofuranosidase, alpha- and beta-D-glucosidase activities are observed. The enzyme hydrolyzes xylooligomers into xylose and catalyzes transxylosilation reactions also in presence of alcohols as acceptors, producing xylo-oligosaccharides and alkyl-xylosides	Anoxybacillus sp. 3M	?	-	?
xylobiose + H2O	-	Anoxybacillus sp. 3M	2 beta-D-xylose	-	?
xylotriose + H2O	-	Anoxybacillus sp. 3M	beta-D-xylose + ?	-	?

Subunits

Subunits	Comment	Organism
homodimer	-	Anoxybacillus sp. 3M

Synonyms

Synonyms	Comment	Organism
AbXyl	-	Anoxybacillus sp. 3M

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
60	65	-	Anoxybacillus sp. 3M

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
55	70	55°C: 83% of maximal activity, 70°C: 41% of maximal activity	Anoxybacillus sp. 3M

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
60	-	half-life: 10 h	Anoxybacillus sp. 3M

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
453.29	-	4-nitrophenyl beta-D-xylopyranoside	pH and temperature not specified in the publication	Anoxybacillus sp. 3M

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
5.5	-	-	Anoxybacillus sp. 3M

pH Range

pH Minimum	pH Maximum	Comment	Organism
4.5	7	pH 4.5: 46% of maximal activity, pH 7.0: 50% of maximal activity	Anoxybacillus sp. 3M

pH Stability

pH Stability	pH Stability Maximum	Comment	Organism
4.5	-	24 h, 78% residual activity	Anoxybacillus sp. 3M
8	-	24 h, 88% residual activity	Anoxybacillus sp. 3M

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
21.25	-	D-xylose	pH and temperature not specified in the publication	Anoxybacillus sp. 3M

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
2322	-	4-nitrophenyl beta-D-xylopyranoside	pH and temperature not specified in the publication	Anoxybacillus sp. 3M

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Release 2023.1 (January 2023)