Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ethanol | ethanol at 5, 10 and 15% (v/v) concentration enhances the enzyme activity | Pseudozyma hubeiensis | |
FeSO4 | about 121.72% activity at 1 mM | Pseudozyma hubeiensis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1-ethyl-3-(3-dimethylaminopropyl) carbodiimide | strong inhibition, 69% residual activity at 200 mM | Pseudozyma hubeiensis | |
5-bromosuccinimide | strong inhibition, 84.7% complete inhibition at 0.1 mM | Pseudozyma hubeiensis | |
Ag+ | 93.31% residual activity at 10 mM | Pseudozyma hubeiensis | |
Ca2+ | 84.91% residual activity at 10 mM | Pseudozyma hubeiensis | |
Co2+ | 74.96% residual activity at 10 mM | Pseudozyma hubeiensis | |
Cu2+ | 89.26% residual activity at 10 mM | Pseudozyma hubeiensis | |
D-xylose | competitive inhibition | Pseudozyma hubeiensis | |
EDTA | 73.09% residual activity at 10 mM | Pseudozyma hubeiensis | |
Hg2+ | 86.23% residual activity at 0.1 mM | Pseudozyma hubeiensis | |
Mg2+ | 88.47% residual activity at 10 mM | Pseudozyma hubeiensis | |
Mn2+ | 89.52% residual activity at 10 mM | Pseudozyma hubeiensis | |
additional information | not inhibited by diethyl dicarbonate, phenylmethyl sulfonide, phenyl-glyoxal, N-ethylmaleimide, iodoacetate, citraconic anhydride, and trinotrobenzene sulfonic acid. Ethanol has no effect on enzyme activity at 20% (v/v). | Pseudozyma hubeiensis | |
N-Acetylimidazole | strong inhibition, 62% residual activity at 50 mM | Pseudozyma hubeiensis | |
Ni2+ | 84.7% residual activity at 10 mM | Pseudozyma hubeiensis | |
Pb2+ | 89.22% residual activity at 0.1 mM | Pseudozyma hubeiensis | |
Zn2+ | 91.75% residual activity at 10 mM | Pseudozyma hubeiensis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.537 | - |
4-nitrophenyl beta-D-xylopyranoside | at pH 4.5 and 60°C | Pseudozyma hubeiensis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe3+ | about 110.32% activity at 0.1 mM | Pseudozyma hubeiensis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudozyma hubeiensis | - |
- |
- |
Pseudozyma hubeiensis NCIM 3574 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, QAE-Sephadex A50 ion exchange column chromatography, and Superdex 200 gel filtration | Pseudozyma hubeiensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl beta-D-xylopyranoside + H2O | highest activity | Pseudozyma hubeiensis | 4-nitrophenol + beta-D-xylopyranose | - |
? | |
4-nitrophenyl beta-D-xylopyranoside + H2O | highest activity | Pseudozyma hubeiensis NCIM 3574 | 4-nitrophenol + beta-D-xylopyranose | - |
? | |
additional information | no activity with 4-nitrophenyl beta-glucopyranoside and 4-nitrophenyl alpha-L-arabinopyranoside | Pseudozyma hubeiensis | ? | - |
? | |
additional information | no activity with 4-nitrophenyl beta-glucopyranoside and 4-nitrophenyl alpha-L-arabinopyranoside | Pseudozyma hubeiensis NCIM 3574 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 2 * 110000, SDS-PAGE | Pseudozyma hubeiensis |
monomer | 2 * 112300, MALDI-TOF mass spectrometry | Pseudozyma hubeiensis |
Synonyms | Comment | Organism |
---|---|---|
beta-xylosidase | - |
Pseudozyma hubeiensis |
Xyl | - |
Pseudozyma hubeiensis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Pseudozyma hubeiensis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 70 | about 60% activity at 50°C, 100% activity at 60°C, about 90% activity at 70°C, about 10% activity at 75°C | Pseudozyma hubeiensis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
the enzyme is stable at 50°C for 4 h | Pseudozyma hubeiensis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
- |
Pseudozyma hubeiensis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 6 | about 60% activity at pH 3.0, about 80% activity at pH 4.0, 100% activity at pH 4.5, about 90% activity at pH 5.0, about 45% activity at pH 6.0 | Pseudozyma hubeiensis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3 | 9 | the enzyme is stable at pH range 4.0-9.0 for 4 h and retains 75 and 100% activity at pH 3.0 and 9.0, respectively, after incubation for 24 h | Pseudozyma hubeiensis |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
75 | - |
at pH 4.5 and 60°C | Pseudozyma hubeiensis | D-xylose |