Any feedback?
Literature summary for 3.2.1.37 extracted from
- Putative endoglucanase PcGH5 from Phanerochaete chrysosporium is a beta-xylosidase that cleaves xylans in synergistic action with endo-xylanase (2015), J. Biosci. Bioeng., 119, 416-420 .
Application
| Application | Comment | Organism |
|---|---|---|
| degradation | enzyme increases reducing sugar release of birchwood xylan, beechwood xylan, and arabinoxylan by 6.4%, 13%, 15.8%, respectively, in synergistic action with endoxylanase. The late addition of the enzyme into reaction with endoxylanase results in a larger increase of reducing sugar release from pretreated barley straw that addition at the start or by treatment with endoxylanases alone. The increases observed are 6.3% and 13.8%, respectively | Phanerodontia chrysosporium |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Pichia pastoris | Phanerodontia chrysosporium |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Phanerodontia chrysosporium | - |
- |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Phanerodontia chrysosporium | W8QRE4 | - |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 25 | - |
pH 5.5, 50°C | Phanerodontia chrysosporium |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl beta-D-xylopyranoside + H2O | - |
Phanerodontia chrysosporium | 4-nitrophenol + D-xylose | - |
? |  |
| additional information | no substrates: 4-nitrophenyl-beta-D-glucopyranoside, 4-nitrophenyl-alpha-D-xylopyranoside, 4-nitrophenyl-alpha-L-arabinofuranoside, 4-nitrophenyl-alpha-D-mannopyranoside, 4-nitrophenyl-beta-D-mannoside, carboxymethylcellulose, barley glucan, glucomannan, laminarin. Very poor substrates: xyloglucan, locust bean gum, xylans. Enzyme can perform transglycosylation of xylose and alcohol | Phanerodontia chrysosporium | ? | - |
? | |
| xylobiose + H2O | - |
Phanerodontia chrysosporium | 2 D-xylose | - |
? | |
| xylopentaose + 4 H2O | - |
Phanerodontia chrysosporium | 5 D-xylose | - |
? | |
| xylotriose + 2 H2O | - |
Phanerodontia chrysosporium | 3 D-xylose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 54000, SDS-PAGE of recombinant protein carrying an alpha secretion signal peptide | Phanerodontia chrysosporium |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Xyl5 | - |
Phanerodontia chrysosporium |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Phanerodontia chrysosporium |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
stable up to | Phanerodontia chrysosporium |
| 50 | - |
12 h, 50% residual activity | Phanerodontia chrysosporium |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
- |
Phanerodontia chrysosporium |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
65% residual activity | Phanerodontia chrysosporium |
| 6 | - |
80% residual activity | Phanerodontia chrysosporium |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 8 | 12 h, more than 80% residual activity | Phanerodontia chrysosporium |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
