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Literature summary for 3.2.1.37 extracted from

  • Hyun, Y.J.; Kim, B.; Kim, D.H.
    Cloning and characterization of ginsenoside Ra1-hydrolyzing beta-D-xylosidase from Bifidobacterium breve K-110 (2012), J. Microbiol. Biotechnol., 22, 535-540.
    View publication on PubMed
Search for more literature for 3.2.1.37

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Bifidobacterium breve

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.05
-
 kakkalide in sodium phosphate buffer (pH 7.0), at 37°C Bifidobacterium breve
0.4
-
 ginsenoside Ra1 in sodium phosphate buffer (pH 7.0), at 37°C Bifidobacterium breve
1.45
-
 4-nitrophenyl beta-D-xylopyranoside in sodium phosphate buffer (pH 7.0), at 37°C Bifidobacterium breve

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55700
-
 x * 55700, SDS-PAGE Bifidobacterium breve

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ginsenoside Ra1 + H2O Bifidobacterium breve
-
 ginsenoside Rb2 + D-xylose
-
 ?
ginsenoside Ra1 + H2O Bifidobacterium breve K-110
-
 ginsenoside Rb2 + D-xylose
-
 ?

Organism

Organism UniProt Comment Textmining
Bifidobacterium breve B1P195
-
-
Bifidobacterium breve K-110 B1P195
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni2+-affinity column chromatography Bifidobacterium breve

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.031
-
 crude extract, in sodium phosphate buffer (pH 7.0), at 37°C Bifidobacterium breve
3.32
-
 purified enzyme, in sodium phosphate buffer (pH 7.0), at 37°C Bifidobacterium breve

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl beta-D-xylopyranoside + H2O
-
 Bifidobacterium breve 4-nitrophenol + beta-D-xylopyranose
-
 ?
4-nitrophenyl beta-D-xylopyranoside + H2O
-
 Bifidobacterium breve K-110 4-nitrophenol + beta-D-xylopyranose
-
 ?
ginsenoside Ra1 + H2O
-
 Bifidobacterium breve ginsenoside Rb2 + D-xylose
-
 ?
ginsenoside Ra1 + H2O
-
 Bifidobacterium breve K-110 ginsenoside Rb2 + D-xylose
-
 ?
kakkalide + H2O
-
 Bifidobacterium breve kakkalidone + D-xylose
-
 ?
kakkalide + H2O
-
 Bifidobacterium breve K-110 kakkalidone + D-xylose
-
 ?
additional information the enzyme does not act on 4-nitrophenyl alpha-L-arabinofuranoside, 4-nitrophenyl beta-D-glucopyranoside, or 4-nitrophenyl beta-D-fucopyranoside Bifidobacterium breve ?
-
 ?
additional information the enzyme does not act on 4-nitrophenyl alpha-L-arabinofuranoside, 4-nitrophenyl beta-D-glucopyranoside, or 4-nitrophenyl beta-D-fucopyranoside Bifidobacterium breve K-110 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 55700, SDS-PAGE Bifidobacterium breve

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Bifidobacterium breve

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 50 the enzyme activity is stable within the temperature range 40 to 50°C, but is rapidly lost above 50°C Bifidobacterium breve

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Bifidobacterium breve