Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ethanol | 5% (v/v), 316.9% activity compared to activity without addition of ethanol, 1.25 mM 4-nitrophenyl-beta-D-xylopyranoside as substrate, pH 4.6, 42°C | uncultured rumen bacterium | |
methanol | 5% (v/v), 167% activity compared to activity without addition of methanol, 1.25 mM 4-nitrophenyl-beta-D-xylopyranoside as substrate, pH 4.6, 42°C | uncultured rumen bacterium |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli and purified | uncultured rumen bacterium |
Crystallization (Comment) | Organism |
---|---|
molecular docking studies for the enzyme with the substrates xylobiose and 4-nitrophenyl-beta-D-xylopyranoside. The essential catalytic residues of RuBG3A are D297 and E499. Besides D113, K218, H219, and R185 also form hydrogen bonds with substrates and are condervative. The active center for beta-glucosidic and beta-xylosidic substrates are the same and the bifunctional activity should come from one functional domain. RuBG3A and RuBG3B exhibit higher scores in the docking analysis against 4-nitrophenyl-beta-D-glucopyranoside than against 4-nitrophenyl-beta-D-xylopyranoside, indicating a larger affinity between the enzymes and 4-nitrophenyl-beta-D-glucopyranoside, which is consistent with the kinetic parameters | uncultured rumen bacterium |
molecular docking studies for the enzyme with the substrates xylobiose and 4-nitrophenyl-beta-D-xylopyranoside. The essential catalytic residues of RuBG3B are D272 and E481. Besides D88, K193, H194, and R160 also form hydrogen bonds with substrates and are condervative. The active center for beta-glucosidic and beta-xylosidic substrates are the same and the bifunctional activity should come from one functional domain. RuBG3A and RuBG3B exhibit higher scores in the docking analysis against 4-nitrophenyl-beta-D-glucopyranoside than against 4-nitrophenyl-beta-D-xylopyranoside, indicating a larger affinity between the enzymes and 4-nitrophenyl-beta-D-glucopyranoside, which is consistent with the kinetic parameters | uncultured rumen bacterium |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1317 | - |
4-nitrophenyl beta-D-xylopyranoside | pH 4.6, 42°C | uncultured rumen bacterium | |
65 | - |
4-nitrophenyl beta-D-xylopyranoside | pH 4.6, 40°C | uncultured rumen bacterium |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
CaCl2 | 5 mM, 92.8% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-xylopyranoside as substrate, pH 4.6, 42°C | uncultured rumen bacterium | |
CaCl2 | 5 mM, 99% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-xylopyranoside as substrate, pH 4.6, 40°C | uncultured rumen bacterium | |
CoCl2 | 5 mM, 89.1% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-xylopyranoside as substrate, pH 4.6, 40°C | uncultured rumen bacterium | |
CoCl2 | 5 mM, 91.3% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-xylopyranoside as substrate, pH 4.6, 42°C | uncultured rumen bacterium | |
CuCl2 | 5 mM, 87.8% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-xylopyranoside as substrate, pH 4.6, 40°C | uncultured rumen bacterium | |
CuCl2 | 5 mM, 96.4% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-xylopyranoside as substrate, pH 4.6, 42°C | uncultured rumen bacterium | |
D-fructose | 5 mM, 76.8% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-xylopyranoside as substrate, pH 4.6, 42°C | uncultured rumen bacterium | |
D-fructose | 5 mM, 86.6% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-xylopyranoside as substrate, pH 4.6, 40°C | uncultured rumen bacterium | |
D-glucose | 5 mM, 4.3% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-xylopyranoside as substrate, pH 4.6, 40°C | uncultured rumen bacterium | |
D-glucose | 5 mM, 54.4% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-xylopyranoside as substrate, pH 4.6, 42°C | uncultured rumen bacterium | |
D-xylose | 5 mM, 81.8% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-xylopyranoside as substrate, pH 4.6, 40°C | uncultured rumen bacterium | |
D-xylose | 5 mM, 97.4% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-xylopyranoside as substrate, pH 4.6, 42°C | uncultured rumen bacterium | |
MgCl2 | 5 mM, 87.6% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-xylopyranoside as substrate, pH 4.6, 42°C | uncultured rumen bacterium | |
MgCl2 | 5 mM, 94.8% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-xylopyranoside as substrate, pH 4.6, 40°C | uncultured rumen bacterium | |
MnCl2 | 5 mM, 93.2% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-xylopyranoside as substrate, pH 4.6, 42°C | uncultured rumen bacterium | |
MnCl2 | 5 mM, 98.5% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-xylopyranoside as substrate, pH 4.6, 40°C | uncultured rumen bacterium | |
NiCl2 | 5 mM, 96.3% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-xylopyranoside as substrate, pH 4.6, 42°C | uncultured rumen bacterium | |
NiCl2 | 5 mM, 98.5% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-xylopyranoside as substrate, pH 4.6, 40°C | uncultured rumen bacterium | |
ZnSO4 | 5 mM, 101.1% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-xylopyranoside as substrate, pH 4.6, 40°C | uncultured rumen bacterium | |
ZnSO4 | 5 mM, 99.1% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-xylopyranoside as substrate, pH 4.6, 42°C | uncultured rumen bacterium |
Organic Solvent | Comment | Organism |
---|---|---|
Ethanol | 5% (v/v), 112.7% activity compared to activity without addition of ethanol, 1.25 mM 4-nitrophenyl-beta-D-xylopyranoside as substrate, pH 4.6, 40°C | uncultured rumen bacterium |
Ethanol | 5% (v/v), 316.9% activity compared to activity without addition of ethanol, 1.25 mM 4-nitrophenyl-beta-D-xylopyranoside as substrate, pH 4.6, 42°C | uncultured rumen bacterium |
Methanol | 5% (v/v), 167% activity compared to activity without addition of methanol, 1.25 mM 4-nitrophenyl-beta-D-xylopyranoside as substrate, pH 4.6, 42°C | uncultured rumen bacterium |
Methanol | 5% (v/v), 94.3% activity compared to activity without addition of methanol, 1.25 mM 4-nitrophenyl-beta-D-xylopyranoside as substrate, pH 4.6, 40°C | uncultured rumen bacterium |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
uncultured rumen bacterium | G3BJX3 | Rubg3A, together with Rubg3B, beta-glucosidase/xylosidase activity, EC 3.2.1.37 and 3.2.1.74; isolated from rumen from a yak rumen metagenomic library, chinese yak, Bos grunniens | - |
uncultured rumen bacterium | G3BJX4 | Rubg3B, together with Rubg3A, beta-glucosidase/xylosidase activity, EC 3.2.1.37 and 3.2.1.74; isolated from rumen from a yak rumen metagenomic library, chinese yak, Bos grunniens | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | uncultured rumen bacterium |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | isolated from rumen from a yak rumen metagenomic library, chinese yak, Bos grunniens | uncultured rumen bacterium | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
5.5 | - |
4-nitrophenyl beta-D-xylopyranoside as substrate, pH 4.6, 40°C | uncultured rumen bacterium |
12.1 | - |
4-nitrophenyl beta-D-xylopyranoside as substrate, pH 4.6, 42°C | uncultured rumen bacterium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl beta-D-xylopyranoside + H2O | 15.7% relative activity compared to the beta-glucosidase activity with 4-nitrophenyl beta-D-glucopyranoside as substrate | uncultured rumen bacterium | 4-nitrophenol + D-xylose | - |
? | |
4-nitrophenyl beta-D-xylopyranoside + H2O | 21.1% relative activity compared to the beta-glucosidase activity with 4-nitrophenyl beta-D-glucopyranoside as substrate | uncultured rumen bacterium | 4-nitrophenol + D-xylose | - |
? | |
additional information | RuBG3A or RuBG3B alone do not release any reducing sugars from xylan. The addition of each enzyme into the reaction mixture of xylanase XynR8 improves the liberation of reducing sugars. The addition of RuBG3A with an activity ratio of 10:1 (xylosidase/xylanase) increases the liberation to 218% of that in the presence of XynR8 alone | uncultured rumen bacterium | ? | - |
? | |
additional information | RuBG3A or RuBG3B alone do not release any reducing sugars from xylan. The addition of each enzyme into the reaction mixture of xylanase XynR8 improves the liberation of reducing sugars. The addition of RuBG3B with an activity ratio of 5:1 (xylosidase/xylanase) increases the liberation to 169% of that in the presence of XynR8 alone. RuBG3B shows no synergism with xylanase XynR8 at the optimal pH 7.5, but at pH 5.5, indicating that RuBG3B may have different pH preferences between aryl xylosidic substrate 4-nitrophenyl-beta-D-xylopyranoside and xylooligisaccharide xylan | uncultured rumen bacterium | ? | - |
? | |
xylobiose + H2O | - |
uncultured rumen bacterium | 2 D-xylose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
beta-glucosidase/xylosidase | bifunctional, belongs to to the glycoside hydrolase 3 familiy, GH3 | uncultured rumen bacterium |
beta-xylosidase | - |
uncultured rumen bacterium |
Rubg3A | gene name, together with Rubg3B, beta-glucosidase/xylosidase activity, EC 3.2.1.37 and 3.2.1.74 | uncultured rumen bacterium |
Rubg3B | gene name, together with Rubg3A, beta-glucosidase/xylosidase activity, EC 3.2.1.37 and 3.2.1.74 | uncultured rumen bacterium |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
optimal, with 4-nitrophenyl-beta-D-xylopyranoside as substrate | uncultured rumen bacterium |
40 | - |
assay at | uncultured rumen bacterium |
40 | - |
optimal, with 4-nitrophenyl-beta-D-xylopyranoside | uncultured rumen bacterium |
42 | - |
assay at | uncultured rumen bacterium |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
17 | - |
4-nitrophenyl beta-D-xylopyranoside | pH 4.6, 42°C | uncultured rumen bacterium | |
135 | - |
4-nitrophenyl beta-D-xylopyranoside | pH 4.6, 40°C | uncultured rumen bacterium |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.6 | - |
assay at | uncultured rumen bacterium |
5.5 | - |
optimal, with 4-nitrophenyl beta-D-xylopyranoside as substrate | uncultured rumen bacterium |
7.5 | - |
optimal, with 4-nitrophenyl beta-D-xylopyranoside as substrate | uncultured rumen bacterium |
General Information | Comment | Organism |
---|---|---|
physiological function | RuBG3A or RuBG3B alone do not release any reducing sugars from xylan. The addition of each enzyme into the reaction mixture of xylanase XynR8 improves the liberation of reducing sugars. The addition of RuBG3A with an activity ratio of 10:1 (xylosidase/xylanase) increases the liberation to 218% of that in the presence of XynR8 alone | uncultured rumen bacterium |
physiological function | RuBG3A or RuBG3B alone do not release any reducing sugars from xylan. The addition of each enzyme into the reaction mixture of xylanase XynR8 improves the liberation of reducing sugars. The addition of RuBG3B with an activity ratio of 5:1 (xylosidase/xylanase) increases the liberation to 169% of that in the presence of XynR8 alone. RuBG3B shows no synergism with xylanase XynR8 at the optimal pH 7.5, but at pH 5.5, indicating that RuBG3B may have different pH preferences between aryl xylosidic substrate 4-nitrophenyl-beta-D-xylopyranoside and xylooligisaccharide xylan | uncultured rumen bacterium |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2084 | - |
4-nitrophenyl beta-D-xylopyranoside | pH 4.6, 40°C | uncultured rumen bacterium | |
54080 | - |
4-nitrophenyl beta-D-xylopyranoside | pH 4.6, 42°C | uncultured rumen bacterium |