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Literature summary for 3.2.1.37 extracted from
- Cell-associated beta-xylosidase from Aureobasidium pullulans ATCC 20524: Purification, properties, and characterization of the encoding gene (2010), J. Biosci. Bioeng., 110, 152-157.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Pichia pastoris | Aureobasidium melanogenum |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3.5 | - |
4-nitrophenyl beta-D-xylopyranoside | pH 3.5, 70°C | Aureobasidium melanogenum |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| soluble | - |
Aureobasidium melanogenum | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 84045 | - |
x * 88500, SDS-PAGE, x * 84045, calculated | Aureobasidium melanogenum |
| 88500 | - |
x * 88500, SDS-PAGE, x * 84045, calculated | Aureobasidium melanogenum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aureobasidium melanogenum | D4AHT5 | grown on oat spelt xylan | - |
| Aureobasidium melanogenum ATCC 20524 | D4AHT5 | grown on oat spelt xylan | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | protein is N-glycosylated, 6% carbohydrate contnet. Presence of 16 putative N-glycosylation sites | Aureobasidium melanogenum |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Aureobasidium melanogenum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl alpha-L-arabinofuranoside + H2O | 38.5% of the activity with 4-nitrophenyl beta-D-xylopyranoside | Aureobasidium melanogenum | 4-nitrophenol + alpha-L-arabinofuranose | - |
? | |
| 4-nitrophenyl alpha-L-arabinofuranoside + H2O | 38.5% of the activity with 4-nitrophenyl beta-D-xylopyranoside | Aureobasidium melanogenum ATCC 20524 | 4-nitrophenol + alpha-L-arabinofuranose | - |
? | |
| 4-nitrophenyl beta-D-xylopyranoside + H2O | - |
Aureobasidium melanogenum | 4-nitrophenol + beta-D-xylopyranose | - |
? | |
| 4-nitrophenyl beta-D-xylopyranoside + H2O | - |
Aureobasidium melanogenum ATCC 20524 | 4-nitrophenol + beta-D-xylopyranose | - |
? | |
| additional information | no substrate: 4-nitrophenyl-alpha-D-xylopyranoside, 4-nitrophenyl-beta-D-glucopyranoside, 4-nitrophenyl-alpha-D-glucopyranoside, 4-nitrophenyl-beta-D-galactopyranoside, 4-nitrophenyl-alpha-D-galactopyranoside, 4-nitrophenyl-alpha-L-arabinopyranoside, cellobiose, oat spelt xylan, birch wood xylan, carboxymethyl cellulose, avicel, and soluble starch | Aureobasidium melanogenum | ? | - |
? | |
| additional information | no substrate: 4-nitrophenyl-alpha-D-xylopyranoside, 4-nitrophenyl-beta-D-glucopyranoside, 4-nitrophenyl-alpha-D-glucopyranoside, 4-nitrophenyl-beta-D-galactopyranoside, 4-nitrophenyl-alpha-D-galactopyranoside, 4-nitrophenyl-alpha-L-arabinopyranoside, cellobiose, oat spelt xylan, birch wood xylan, carboxymethyl cellulose, avicel, and soluble starch | Aureobasidium melanogenum ATCC 20524 | ? | - |
? | |
| xylobiose + H2O | 30.8% of the activity with 4-nitrophenyl beta-D-xylopyranoside | Aureobasidium melanogenum | 2 beta-D-xylopyranose | - |
? | |
| xylobiose + H2O | 30.8% of the activity with 4-nitrophenyl beta-D-xylopyranoside | Aureobasidium melanogenum ATCC 20524 | 2 beta-D-xylopyranose | - |
? | |
| xylotriose + 2 H2O | 32.8% of the activity with 4-nitrophenyl beta-D-xylopyranoside | Aureobasidium melanogenum | 3 beta-D-xylopyranose | - |
? | |
| xylotriose + 2 H2O | 32.8% of the activity with 4-nitrophenyl beta-D-xylopyranoside | Aureobasidium melanogenum ATCC 20524 | 3 beta-D-xylopyranose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 88500, SDS-PAGE, x * 84045, calculated | Aureobasidium melanogenum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
- |
Aureobasidium melanogenum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
stable up to 30 min | Aureobasidium melanogenum |
| 80 | - |
30 min, loss of activity | Aureobasidium melanogenum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 3.5 | - |
- |
Aureobasidium melanogenum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
significant decrease in activity above | Aureobasidium melanogenum |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 9 | 3 h, 70% of iniital activity | Aureobasidium melanogenum |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Aureobasidium melanogenum | calculated | - |
4.6 |
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