Protein Variants | Comment | Organism |
---|---|---|
additional information | with use of vector pHsh, expression of XylC increases approximately 4fold when a loop within the translational initiation region in the mRNA is removed by site-directed mutagenesis. The increased expression of xylCm is due to removal of a stem-loop structure without a change of the amino acid sequence of the heterologously expressed enzyme | Thermoanaerobacterium saccharolyticum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | EDTA is not inhibitory up to 10 mM | Thermoanaerobacterium saccharolyticum | |
xylose | not inhibitory up to 70 mM, 70% of its activity is retained in the presence of 200 mM xylose | Thermoanaerobacterium saccharolyticum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
28 | - |
4-nitrophenyl-beta-D-xylopyranoside | pH 6.0, 65°C | Thermoanaerobacterium saccharolyticum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
78000 | - |
x * 78000, SDS-PAGE | Thermoanaerobacterium saccharolyticum |
86000 | - |
x * 86000, SDS-PAGE | Thermoanaerobacterium saccharolyticum |
112000 | - |
PAGE | Thermoanaerobacterium saccharolyticum |
122000 | - |
gel filtration | Thermoanaerobacterium saccharolyticum |
165000 | - |
PAGE | Thermoanaerobacterium saccharolyticum |
210000 | - |
gel filtration | Thermoanaerobacterium saccharolyticum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermoanaerobacterium saccharolyticum | - |
- |
- |
Thermoanaerobacterium saccharolyticum | A2ICH1 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | 6% carbohydrate content | Thermoanaerobacterium saccharolyticum |
no glycoprotein | - |
Thermoanaerobacterium saccharolyticum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
45.8 | - |
pH 6.0, 65°C | Thermoanaerobacterium saccharolyticum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl beta-D-xylopyranoside + H2O | - |
Thermoanaerobacterium saccharolyticum | 4-nitrophenol + beta-D-xylose | - |
? | |
additional information | enzyme produces beta-xylose from beta-xylopyranosides while retaining the anomeric configuration of the xylose in the substrate. Enzyme shows transglycosylation activities in the presence of alcohols as acceptors. No substrate: 4-nitrophenyl-alpha-L-arabinofuranoside, 4-nitrophenyl-alpha-D-xylopyranoside, 4-nitrophenyl-alpha-D-glucopyranoside, and 4-nitrophenyl-beta-D-glucopyranoside, oat spelt xylan, birch wood xylan, or carboxymethyl cellulose | Thermoanaerobacterium saccharolyticum | ? | - |
? | |
xylobiose + H2O | - |
Thermoanaerobacterium saccharolyticum | 2 beta-D-xylose | - |
? | |
xylotriose + 2 H2O | - |
Thermoanaerobacterium saccharolyticum | 3 beta-D-xylose | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 78000, SDS-PAGE | Thermoanaerobacterium saccharolyticum |
? | x * 86000, SDS-PAGE | Thermoanaerobacterium saccharolyticum |
Synonyms | Comment | Organism |
---|---|---|
XYLA | - |
Thermoanaerobacterium saccharolyticum |
XylC | - |
Thermoanaerobacterium saccharolyticum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
- |
Thermoanaerobacterium saccharolyticum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
67 | - |
half-life 1 h | Thermoanaerobacterium saccharolyticum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
189.3 | - |
4-nitrophenyl-beta-D-xylopyranoside | pH 6.0, 65°C | Thermoanaerobacterium saccharolyticum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Thermoanaerobacterium saccharolyticum |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 8 | stable within | Thermoanaerobacterium saccharolyticum |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Thermoanaerobacterium saccharolyticum | isoelectric focusing | - |
4.4 |
Thermoanaerobacterium saccharolyticum | isoelectric focusing | - |
4.5 |