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Literature summary for 3.2.1.37 extracted from

  • Shao, W.; Xue, Y.; Wu, A.; Kataeva, I.; Pei, J.; Wu, H.; Wiegel, J.
    Characterization of a novel beta-xylosidase, XylC, from Thermoanaerobacterium saccharolyticum JW/SL-YS485 (2011), Appl. Environ. Microbiol., 77, 719-726.
    View publication on PubMedView publication on EuropePMC

Protein Variants

Protein Variants Comment Organism
additional information with use of vector pHsh, expression of XylC increases approximately 4fold when a loop within the translational initiation region in the mRNA is removed by site-directed mutagenesis. The increased expression of xylCm is due to removal of a stem-loop structure without a change of the amino acid sequence of the heterologously expressed enzyme Thermoanaerobacterium saccharolyticum

Inhibitors

Inhibitors Comment Organism Structure
additional information EDTA is not inhibitory up to 10 mM Thermoanaerobacterium saccharolyticum
xylose not inhibitory up to 70 mM, 70% of its activity is retained in the presence of 200 mM xylose Thermoanaerobacterium saccharolyticum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
28
-
4-nitrophenyl-beta-D-xylopyranoside pH 6.0, 65°C Thermoanaerobacterium saccharolyticum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
78000
-
x * 78000, SDS-PAGE Thermoanaerobacterium saccharolyticum
86000
-
x * 86000, SDS-PAGE Thermoanaerobacterium saccharolyticum
112000
-
PAGE Thermoanaerobacterium saccharolyticum
122000
-
gel filtration Thermoanaerobacterium saccharolyticum
165000
-
PAGE Thermoanaerobacterium saccharolyticum
210000
-
gel filtration Thermoanaerobacterium saccharolyticum

Organism

Organism UniProt Comment Textmining
Thermoanaerobacterium saccharolyticum
-
-
-
Thermoanaerobacterium saccharolyticum A2ICH1
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein 6% carbohydrate content Thermoanaerobacterium saccharolyticum
no glycoprotein
-
Thermoanaerobacterium saccharolyticum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
45.8
-
pH 6.0, 65°C Thermoanaerobacterium saccharolyticum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl beta-D-xylopyranoside + H2O
-
Thermoanaerobacterium saccharolyticum 4-nitrophenol + beta-D-xylose
-
?
additional information enzyme produces beta-xylose from beta-xylopyranosides while retaining the anomeric configuration of the xylose in the substrate. Enzyme shows transglycosylation activities in the presence of alcohols as acceptors. No substrate: 4-nitrophenyl-alpha-L-arabinofuranoside, 4-nitrophenyl-alpha-D-xylopyranoside, 4-nitrophenyl-alpha-D-glucopyranoside, and 4-nitrophenyl-beta-D-glucopyranoside, oat spelt xylan, birch wood xylan, or carboxymethyl cellulose Thermoanaerobacterium saccharolyticum ?
-
?
xylobiose + H2O
-
Thermoanaerobacterium saccharolyticum 2 beta-D-xylose
-
?
xylotriose + 2 H2O
-
Thermoanaerobacterium saccharolyticum 3 beta-D-xylose
-
?

Subunits

Subunits Comment Organism
? x * 78000, SDS-PAGE Thermoanaerobacterium saccharolyticum
? x * 86000, SDS-PAGE Thermoanaerobacterium saccharolyticum

Synonyms

Synonyms Comment Organism
XYLA
-
Thermoanaerobacterium saccharolyticum
XylC
-
Thermoanaerobacterium saccharolyticum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
-
Thermoanaerobacterium saccharolyticum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
67
-
half-life 1 h Thermoanaerobacterium saccharolyticum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
189.3
-
4-nitrophenyl-beta-D-xylopyranoside pH 6.0, 65°C Thermoanaerobacterium saccharolyticum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
Thermoanaerobacterium saccharolyticum

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 8 stable within Thermoanaerobacterium saccharolyticum

pI Value

Organism Comment pI Value Maximum pI Value
Thermoanaerobacterium saccharolyticum isoelectric focusing
-
4.4
Thermoanaerobacterium saccharolyticum isoelectric focusing
-
4.5