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Literature summary for 3.2.1.37 extracted from
- Novel bifunctional alpha-L-arabinofuranosidase/xylobiohydrolase (ABF3) from Penicillium purpurogenum (2010), Appl. Environ. Microbiol., 76, 5247-5253.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.65 | - |
4-nitrophenyl alpha-L-arabinopyranoside | pH 4.0, 30°C | Talaromyces purpureogenus |  |
| 12 | - |
4-nitrophenyl beta-D-xyloside | pH 4.0, 30°C | Talaromyces purpureogenus | |
| 12 | - |
4-nitrophenyl alpha-D-xylopyranoside | in 50 mM sodium citrate buffer (pH 4.0), at 30°C | Talaromyces purpureogenus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 28000 | - |
gel filtration | Talaromyces purpureogenus |
| 47305 | - |
1 * 47305, mature protein, calculated from amino acid sequence | Talaromyces purpureogenus |
| 47305 | - |
1 * 50700, SDS-PAGE, 1 * 47305, calculated | Talaromyces purpureogenus |
| 50700 | - |
1 * 50700, deduced from SDS-PAGE | Talaromyces purpureogenus |
| 50700 | - |
1 * 50700, SDS-PAGE, 1 * 47305, calculated | Talaromyces purpureogenus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Talaromyces purpureogenus | C3W4Y2 | bifunctional alpha-L-arabinofuranosidase/xylobiohydrolase | - |
| Talaromyces purpureogenus | C3W4Y2 | soft rot fungus | - |
| Talaromyces purpureogenus ATCC MYA-38 | C3W4Y2 | bifunctional alpha-L-arabinofuranosidase/xylobiohydrolase | - |
| Talaromyces purpureogenus ATCC MYA-38 | C3W4Y2 | soft rot fungus | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | - |
Talaromyces purpureogenus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Talaromyces purpureogenus |
| ammonium sulfate precipitation, DEAE-Sephadex gel filtration, SP Sepharose column chromatography and BioGel P-300 gel filtration | Talaromyces purpureogenus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 89.5 | - |
pH 4.0, 30°C, substrate 4-nitrophenyl alpha-L-arabinopyranoside | Talaromyces purpureogenus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl alpha-D-xylopyranoside + H2O | - |
Talaromyces purpureogenus | 4-nitrophenol + D-xylopyranose | - |
? | |
| 4-nitrophenyl alpha-D-xylopyranoside + H2O | - |
Talaromyces purpureogenus ATCC MYA-38 | 4-nitrophenol + D-xylopyranose | - |
? | |
| 4-nitrophenyl alpha-L-arabinopyranoside + H2O | - |
Talaromyces purpureogenus | 4-nitrophenol + alpha-L-arabinopyranose | less than 2% activity with 4-nitrophenyl alpha-L-arabinopyranoside | ? | |
| 4-nitrophenyl alpha-L-arabinopyranoside + H2O | - |
Talaromyces purpureogenus ATCC MYA-38 | 4-nitrophenol + alpha-L-arabinopyranose | less than 2% activity with 4-nitrophenyl alpha-L-arabinopyranoside | ? | |
| 4-nitrophenyl beta-D-xyloside + H2O | - |
Talaromyces purpureogenus | 4-nitrophenol + beta-D-xylose | - |
? | |
| 4-nitrophenyl beta-D-xyloside + H2O | - |
Talaromyces purpureogenus ATCC MYA-38 | 4-nitrophenol + beta-D-xylose | - |
? | |
| additional information | less than 2% activity with 4-nitrophenyl alpha-L-arabinopyranoside, 2-nitrophenyl beta-D-xylopyranoside, 4-nitrophenyl beta-D-glucopyranoside, 4-nitrophenyl beta-D-mannopyranoside, 4-nitrophenyl alpha-D-glucopyranoside, 4-nitrophenyl beta-D-fucopyranoside, 4-nitrophenyl alpha-D-galactopyranoside, and 4-nitrophenyl beta-D-galctopyranoside. Only small amounts of xylose are released from oligosaccharides. Smaller oligosaccharides are produced when xylotriose through xylopentaose are used as substrates, suggesting a possible xylobiohydrolase activity. No transglycosylation is detected | Talaromyces purpureogenus | ? | - |
? | |
| additional information | less than 2% activity with 4-nitrophenyl alpha-L-arabinopyranoside, 2-nitrophenyl beta-D-xylopyranoside, 4-nitrophenyl beta-D-glucopyranoside, 4-nitrophenyl beta-D-mannopyranoside, 4-nitrophenyl alpha-D-glucopyranoside, 4-nitrophenyl beta-D-fucopyranoside, 4-nitrophenyl alpha-D-galactopyranoside, and 4-nitrophenyl beta-D-galctopyranoside. Only small amounts of xylose are released from oligosaccharides. Smaller oligosaccharides are produced when xylotriose through xylopentaose are used as substrates, suggesting a possible xylobiohydrolase activity. No transglycosylation is detected | Talaromyces purpureogenus ATCC MYA-38 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 47305, mature protein, calculated from amino acid sequence | Talaromyces purpureogenus |
| monomer | 1 * 50700, deduced from SDS-PAGE | Talaromyces purpureogenus |
| monomer | 1 * 50700, SDS-PAGE, 1 * 47305, calculated | Talaromyces purpureogenus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ABF3 | - |
Talaromyces purpureogenus |
| ABF3 | bifunctional enzyme having both alpha-L-arabinofuranosidase and xylobiohydrolase functionalities | Talaromyces purpureogenus |
| alpha-L-arabinofuranosidase 3 | - |
Talaromyces purpureogenus |
| alpha-L-arabinofuranoside arabinofuranohydrolase | - |
Talaromyces purpureogenus |
| arabinofuranosidase 3 | bifunctional enzyme having both alpha-L-arabinofuranosidase and xylobiohydrolase functionalities | Talaromyces purpureogenus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Talaromyces purpureogenus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
30 min, 35% loss of activity | Talaromyces purpureogenus |
| 35 | 50 | the enzyme loses 40% of its activity at 35°C and 90% at 50°C after 30 min of incubation | Talaromyces purpureogenus |
| 50 | - |
30 min, 90% loss of activity | Talaromyces purpureogenus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.73 | - |
4-nitrophenyl alpha-L-arabinopyranoside | pH 4.0, 30°C | Talaromyces purpureogenus | |
| 253.3 | - |
4-nitrophenyl alpha-D-xylopyranoside | in 50 mM sodium citrate buffer (pH 4.0), at 30°C | Talaromyces purpureogenus | |
| 253.3 | - |
4-nitrophenyl beta-D-xyloside | pH 4.0, 30°C | Talaromyces purpureogenus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Talaromyces purpureogenus |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 4.21 | - |
4-nitrophenyl alpha-L-arabinopyranoside | pH 4.0, 30°C | Talaromyces purpureogenus | |
| 21.1 | - |
4-nitrophenyl beta-D-xyloside | pH 4.0, 30°C | Talaromyces purpureogenus | |
| 21.7 | - |
4-nitrophenyl alpha-D-xylopyranoside | in 50 mM sodium citrate buffer (pH 4.0), at 30°C | Talaromyces purpureogenus | |
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