Application | Comment | Organism |
---|---|---|
degradation | in hydrolysis of corn stover hemicellulose, the xylose production increases by 94.9% and 140% when Trichoderma reesei hemicellulase is supplemented with purified beta-xylosidase and crude cell wall proteins of corn stover, respectively | Zea mays |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | 10 mM, 42% residual activity | Zea mays | |
Ag+ | 10 mM, 10% residual activity | Zea mays | |
Cu2+ | 10 mM, 22% residual activity | Zea mays | |
EDTA | 10 mM, 36% residual activity | Zea mays | |
Hg2+ | 10 mM, 8% residual activity | Zea mays | |
Ni2+ | 10 mM, 13% residual activity | Zea mays |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.5 | - |
4-nitrophenyl-beta-D-xylopyranoside | pH 5.0, 37°C | Zea mays |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cell wall | - |
Zea mays | 5618 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | 10 mM, 130% of initial activity | Zea mays | |
Li+ | 10 mM, 147% of initial activity | Zea mays | |
Mn2+ | 10 mM, 122% of initial activity | Zea mays | |
Na+ | 10 mM, 126% of initial activity | Zea mays |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
68500 | - |
x * 68500, SDS-PAGE | Zea mays |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Zea mays | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Zea mays |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl beta-D-xylopyranoside + H2O | - |
Zea mays | 4-nitrophenol + beta-D-xylose | - |
? | |
additional information | no substrate: xylan, 4-nitrophenyl-beta-D-cellobiose, 4-nitrophenyl-alpha-L-arabinofuranoside, carboxymethycellulose | Zea mays | ? | - |
? | |
xylobiose + H2O | - |
Zea mays | 2 D-xylose | - |
? | |
xylopentaose + H2O | - |
Zea mays | 5 D-xylose | - |
? | |
xylotetraose + 3 H2O | - |
Zea mays | 4 D-xylose | - |
? | |
xylotriose + H2O | - |
Zea mays | 3 D-xylose | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 68500, SDS-PAGE | Zea mays |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Zea mays |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 50 | - |
Zea mays |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
30 min, 13.6% loss of activity | Zea mays |
50 | - |
30 min, loss of 20% of activity | Zea mays |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6.5 | - |
4-nitrophenyl-beta-D-xylopyranoside | pH 5.0, 37°C | Zea mays |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
- |
Zea mays |