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Literature summary for 3.2.1.37 extracted from

  • Han, Y.; Chen, H.
    A beta-xylosidase from cell wall of maize: Purification, properties and its use in hydrolysis of plant cell wall (2010), J. Mol. Catal. B, 63, 135-140.
No PubMed abstract available

Application

Application Comment Organism
degradation in hydrolysis of corn stover hemicellulose, the xylose production increases by 94.9% and 140% when Trichoderma reesei hemicellulase is supplemented with purified beta-xylosidase and crude cell wall proteins of corn stover, respectively Zea mays

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol 10 mM, 42% residual activity Zea mays
Ag+ 10 mM, 10% residual activity Zea mays
Cu2+ 10 mM, 22% residual activity Zea mays
EDTA 10 mM, 36% residual activity Zea mays
Hg2+ 10 mM, 8% residual activity Zea mays
Ni2+ 10 mM, 13% residual activity Zea mays

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5
-
4-nitrophenyl-beta-D-xylopyranoside pH 5.0, 37°C Zea mays

Localization

Localization Comment Organism GeneOntology No. Textmining
cell wall
-
Zea mays 5618
-

Metals/Ions

Metals/Ions Comment Organism Structure
K+ 10 mM, 130% of initial activity Zea mays
Li+ 10 mM, 147% of initial activity Zea mays
Mn2+ 10 mM, 122% of initial activity Zea mays
Na+ 10 mM, 126% of initial activity Zea mays

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
68500
-
x * 68500, SDS-PAGE Zea mays

Organism

Organism UniProt Comment Textmining
Zea mays
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Zea mays

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl beta-D-xylopyranoside + H2O
-
Zea mays 4-nitrophenol + beta-D-xylose
-
?
additional information no substrate: xylan, 4-nitrophenyl-beta-D-cellobiose, 4-nitrophenyl-alpha-L-arabinofuranoside, carboxymethycellulose Zea mays ?
-
?
xylobiose + H2O
-
Zea mays 2 D-xylose
-
?
xylopentaose + H2O
-
Zea mays 5 D-xylose
-
?
xylotetraose + 3 H2O
-
Zea mays 4 D-xylose
-
?
xylotriose + H2O
-
Zea mays 3 D-xylose
-
?

Subunits

Subunits Comment Organism
? x * 68500, SDS-PAGE Zea mays

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
Zea mays

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 50
-
Zea mays

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
30 min, 13.6% loss of activity Zea mays
50
-
30 min, loss of 20% of activity Zea mays

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.5
-
4-nitrophenyl-beta-D-xylopyranoside pH 5.0, 37°C Zea mays

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
-
Zea mays