Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-nitrophenyl-beta-D-xylopyranoside | - |
Aspergillus japonicus | |
4-nitrophenyl-beta-D-xylopyranoside | - |
Trichoderma reesei | |
xylose | competitive | Aspergillus japonicus | |
xylose | competitive | Trichoderma reesei |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.33 | - |
4-nitrophenyl-beta-D-xylopyranoside | pH 5.0, 40°C | Aspergillus japonicus | |
4.4 | - |
Xyl-beta-(1->4)-Xyl-beta-(1->4)-Xyl-beta-(1->4)-Xyl | pH 5.0, 40°C | Aspergillus japonicus | |
4.6 | - |
Xyl-beta-(1->4)-Xyl | pH 5.0, 40°C | Aspergillus japonicus | |
5.7 | - |
Xyl-beta-(1->4)-Xyl-beta-(1->4)-Xyl | pH 5.0, 40°C | Aspergillus japonicus | |
7.2 | - |
xylobiose | pH 5.0, 40°C | Trichoderma reesei | |
7.2 | - |
xylotriose | pH 5.0, 40°C | Trichoderma reesei | |
7.2 | - |
xylotetraose | pH 5.0, 40°C | Trichoderma reesei | |
12 | - |
4-nitrophenyl-beta-D-xylopyranoside | pH 5.0, 40°C | Trichoderma reesei |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus japonicus | - |
- |
- |
Trichoderma reesei | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.1 | - |
substrate 4-nitrophenyl-alpha-L-arabinofuranoside, pH 5.0, 40°C | Trichoderma reesei |
0.1 | - |
substrate arabinoxylan, pH 5.0, 40!C | Aspergillus japonicus |
0.9 | - |
substrate arabinoxylan, pH 5.0, 40°C | Trichoderma reesei |
3.9 | - |
substrate 4-nitrophenyl-alpha-L-arabinofuranoside, pH 5.0, 40°C | Aspergillus japonicus |
7 | - |
substrate glucuronoxylan, pH 5.0, 40°C | Aspergillus japonicus |
14.2 | - |
substrate glucuronoxylan, pH 5.0, 40°C | Trichoderma reesei |
16 | - |
substrate 4-nitrophenyl-beta-D-xylopyranoside, pH 5.0, 40°C | Trichoderma reesei |
25 | - |
substrate xylobiose, pH 5.0, 40°C | Trichoderma reesei |
28.4 | - |
substrate Xyl-beta-(1->4)-Xyl, pH 5.0, 40°C | Aspergillus japonicus |
42.6 | - |
substrate 4-nitrophenyl-beta-D-xylopyranoside, pH 5.0, 40°C | Aspergillus japonicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl alpha-L-arabinofuranoside + H2O | - |
Trichoderma reesei | 4-nitrophenol + alpha-L-arabinofuranose | - |
? | |
4-nitrophenyl alpha-L-arabinofuranoside + H2O | - |
Aspergillus japonicus | 4-nitrophenol + alpha-L-arabinofuranose | - |
? | |
4-nitrophenyl beta-D-xylopyranoside + H2O | - |
Trichoderma reesei | 4-nitrophenol + beta-D-xylopyranose | - |
? | |
4-nitrophenyl beta-D-xylopyranoside + H2O | - |
Aspergillus japonicus | 4-nitrophenol + beta-D-xylopyranose | - |
? | |
arabinoxylan + H2O | - |
Trichoderma reesei | ? | - |
? | |
arabinoxylan + H2O | - |
Aspergillus japonicus | ? | - |
? | |
glucuronoxylan + H2O | - |
Trichoderma reesei | ? | - |
? | |
glucuronoxylan + H2O | - |
Aspergillus japonicus | ? | - |
? | |
additional information | no substrate: xyloglucan | Trichoderma reesei | ? | - |
? | |
additional information | no substrate: xyloglucan | Aspergillus japonicus | ? | - |
? | |
additional information | the beta-xylosidase exhibits synergism with endoxylanase upon hydrolysis of glucuronoxylan as well as with alpha-L-arabinofuranosidase and endoxylanase upon hydrolysis of arabinoxylan. Addition of beta-xylosidases increases efficiency of hydrolysis of plant raw materials with high hemicellulose content such as maize cobs by the enzymic preparation Celloviridine G20x depleted of its own beta-xylosidase | Trichoderma reesei | ? | - |
? | |
additional information | the beta-xylosidase exhibits synergism with endoxylanase upon hydrolysis of glucuronoxylan as well as with alpha-L-arabinofuranosidase and endoxylanase upon hydrolysis of arabinoxylan. Addition of beta-xylosidases increases efficiency of hydrolysis of plant raw materials with high hemicellulose content such as maize cobs by the enzymic preparation Celloviridine G20x depleted of its own beta-xylosidase | Aspergillus japonicus | ? | - |
? | |
Xyl-beta-(1->4)-Xyl + H2O | - |
Aspergillus japonicus | 2 D-xylose | - |
? | |
Xyl-beta-(1->4)-Xyl-beta-(1->4)-Xyl + H2O | - |
Aspergillus japonicus | 3 D-xylose | - |
? | |
Xyl-beta-(1->4)-Xyl-beta-(1->4)-Xyl-beta-(1->4)-Xyl + H2O | - |
Aspergillus japonicus | 4 D-xylose | - |
? | |
xylobiose + H2O | - |
Trichoderma reesei | 2 D-xylose | - |
? | |
xylotetraose + 3 H2O | - |
Trichoderma reesei | 4 D-xylose | - |
? | |
xylotriose + H2O | - |
Trichoderma reesei | 3 D-xylose | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Trichoderma reesei |
70 | - |
- |
Aspergillus japonicus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
3 h, no loss of activity | Trichoderma reesei |
60 | - |
3 h, no loss of activity | Aspergillus japonicus |
60 | - |
3 h, 52% residual activity | Trichoderma reesei |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
3.5 | - |
- |
Trichoderma reesei |
4 | - |
- |
Aspergillus japonicus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
1.4 | - |
4-nitrophenyl-beta-D-xylopyranoside | pH 5.0, 40°C | Trichoderma reesei | |
2.9 | - |
4-nitrophenyl-beta-D-xylopyranoside | pH 5.0, 40°C | Aspergillus japonicus |
General Information | Comment | Organism |
---|---|---|
physiological function | the beta-xylosidase exhibits synergism with endoxylanase upon hydrolysis of glucuronoxylan as well as with alpha-L-arabinofuranosidase and endoxylanase upon hydrolysis of arabinoxylan. Addition of beta-xylosidases increases efficiency of hydrolysis of plant raw materials with high hemicellulose content such as maize cobs by the enzymic preparation Celloviridine G20x depleted of its own beta-xylosidase | Trichoderma reesei |
physiological function | the beta-xylosidase exhibits synergism with endoxylanase upon hydrolysis of glucuronoxylan as well as with alpha-L-arabinofuranosidase and endoxylanase upon hydrolysis of arabinoxylan. Addition of beta-xylosidases increases efficiency of hydrolysis of plant raw materials with high hemicellulose content such as maize cobs by the enzymic preparation Celloviridine G20x depleted of its own beta-xylosidase | Aspergillus japonicus |