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Literature summary for 3.2.1.37 extracted from

  • Jordan, D.B.; Braker, J.D.
    beta-D-Xylosidase from Selenomonas ruminantium: role of glutamate 186 in catalysis revealed by site-directed mutagenesis, alternate substrates, and active-site inhibitor (2010), Appl. Biochem. Biotechnol., 161, 395-410.
    View publication on PubMed
Search for more literature for 3.2.1.37

Protein Variants

Protein Variants Comment Organism
E186A The E186A mutation abolishes the pKa assigned to the catalytic acid E186, mutant enzyme binds only the neutral aminoalcohol triethanolamine, whereas wild-type enzyme binds only the cationic aminoalcohol. At pH 7.0 and 25°C, the ratio of kcat values for substrates 4-nitrophenyl-beta-D-xylopyranoside and 4-nitrophenyl-alpha-L-arabinofuranoside of E186A is 100fold that of wild-type enzyme Selenomonas ruminantium

Inhibitors

Inhibitors Comment Organism Structure
triethanolamine
-
 Selenomonas ruminantium

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.133
-
 4-nitrophenyl-alpha-L-arabinofuranoside mutant E186A, pH 8.0, 25°C Selenomonas ruminantium
0.152
-
 4-nitrophenyl-alpha-L-arabinofuranoside mutant E186A, pH 7.0, 25°C Selenomonas ruminantium
0.177
-
 4-nitrophenyl-alpha-L-arabinofuranoside mutant E186A, pH 6.0, 25°C Selenomonas ruminantium
0.33
-
 4-nitrophenyl-beta-D-xylopyranoside wild-type, pH 8.0, 25°C Selenomonas ruminantium
0.39
-
 4-nitrophenyl-beta-D-xylopyranoside wild-type, pH 7.0, 25°C Selenomonas ruminantium
0.599
-
 4-nitrophenyl-beta-D-xylopyranoside wild-type, pH 6.0, 25°C Selenomonas ruminantium
0.715
-
 4-nitrophenyl-alpha-L-arabinofuranoside wild-type, pH 6.0, 25°C Selenomonas ruminantium
1.08
-
 4-nitrophenyl-alpha-L-arabinofuranoside wild-type, pH 7.0, 25°C Selenomonas ruminantium
2.57
-
 4-nitrophenyl-beta-D-xylopyranoside mutant E186A, pH 8.0, 25°C Selenomonas ruminantium
2.82
-
 4-nitrophenyl-beta-D-xylopyranoside mutant E186A, pH 6.0, 25°C Selenomonas ruminantium
3.27
-
 4-nitrophenyl-beta-D-xylopyranoside mutant E186A, pH 7.0, 25°C Selenomonas ruminantium
4.09
-
 4-nitrophenyl-alpha-L-arabinofuranoside wild-type, pH 8.0, 25°C Selenomonas ruminantium

Organism

Organism UniProt Comment Textmining
Selenomonas ruminantium
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl alpha-L-arabinofuranoside + H2O
-
 Selenomonas ruminantium 4-nitrophenol + alpha-L-arabinofuranose
-
 ?
4-nitrophenyl beta-D-xylopyranoside + H2O
-
 Selenomonas ruminantium 4-nitrophenol + beta-D-xylopyranose
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0000036
-
 4-nitrophenyl-alpha-L-arabinofuranoside mutant E186A, pH 7.0, 25°C Selenomonas ruminantium
0.0000044
-
 4-nitrophenyl-alpha-L-arabinofuranoside mutant E186A, pH 8.0, 25°C Selenomonas ruminantium
0.0000045
-
 4-nitrophenyl-alpha-L-arabinofuranoside mutant E186A, pH 6.0, 25°C Selenomonas ruminantium
0.0014
-
 4-nitrophenyl-beta-D-xylopyranoside mutant E186A, pH 6.0, 25°C Selenomonas ruminantium
0.0016
-
 4-nitrophenyl-beta-D-xylopyranoside mutant E186A, pH 7.0, 25°C Selenomonas ruminantium
0.0016
-
 4-nitrophenyl-beta-D-xylopyranoside mutant E186A, pH 8.0, 25°C Selenomonas ruminantium
2.36
-
 4-nitrophenyl-beta-D-xylopyranoside wild-type, pH 8.0, 25°C Selenomonas ruminantium
3.05
-
 4-nitrophenyl-alpha-L-arabinofuranoside wild-type, pH 8.0, 25°C Selenomonas ruminantium
3.13
-
 4-nitrophenyl-alpha-L-arabinofuranoside wild-type, pH 6.0, 25°C Selenomonas ruminantium
3.28
-
 4-nitrophenyl-alpha-L-arabinofuranoside wild-type, pH 7.0, 25°C Selenomonas ruminantium
14.4
-
 4-nitrophenyl-beta-D-xylopyranoside wild-type, pH 7.0, 25°C Selenomonas ruminantium
29.3
-
 4-nitrophenyl-beta-D-xylopyranoside wild-type, pH 6.0, 25°C Selenomonas ruminantium

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.088
-
 triethanolamine wild-type, substrate 4-nitrophenyl-beta-D-xylopyranoside, pH 8.0, 25°C Selenomonas ruminantium
0.111
-
 triethanolamine wild-type, substrate 4-nitrophenyl-beta-D-xylopyranoside, pH 7.0, 25°C Selenomonas ruminantium
0.121
-
 triethanolamine wild-type, substrate 4-nitrophenyl-alpha-L-arabinofuranoside, pH 7.0, 25°C Selenomonas ruminantium
0.54
-
 triethanolamine wild-type, substrate 4-nitrophenyl-beta-D-xylopyranoside, pH 6.0, 25°C Selenomonas ruminantium
33.2
-
 triethanolamine mutant E186A, substrate 4-nitrophenyl-beta-D-xylopyranoside, pH 8.0, 25°C Selenomonas ruminantium
47
-
 triethanolamine mutant E186A, substrate 4-nitrophenyl-alpha-L-arabinofuranoside, pH 8.0, 25°C Selenomonas ruminantium
145
-
 triethanolamine mutant E186A, substrate 4-nitrophenyl-beta-D-xylopyranoside, pH 7.0, 25°C Selenomonas ruminantium
418
-
 triethanolamine mutant E186A, substrate 4-nitrophenyl-beta-D-xylopyranoside, pH 6.0, 25°C Selenomonas ruminantium
815
-
 triethanolamine mutant E186A, substrate 4-nitrophenyl-alpha-L-arabinofuranoside, pH 7.0, 25°C Selenomonas ruminantium

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.000024
-
 4-nitrophenyl-alpha-L-arabinofuranoside mutant E186A, pH 7.0, 25°C Selenomonas ruminantium
0.000025
-
 4-nitrophenyl-alpha-L-arabinofuranoside mutant E186A, pH 6.0, 25°C Selenomonas ruminantium
0.000033
-
 4-nitrophenyl-alpha-L-arabinofuranoside mutant E186A, pH 8.0, 25°C Selenomonas ruminantium
0.0005
-
 4-nitrophenyl-beta-D-xylopyranoside mutant E186A, pH 7.0, 25°C Selenomonas ruminantium
0.00051
-
 4-nitrophenyl-beta-D-xylopyranoside mutant E186A, pH 6.0, 25°C Selenomonas ruminantium
0.00062
-
 4-nitrophenyl-beta-D-xylopyranoside mutant E186A, pH 8.0, 25°C Selenomonas ruminantium
0.746
-
 4-nitrophenyl-alpha-L-arabinofuranoside wild-type, pH 8.0, 25°C Selenomonas ruminantium
3.03
-
 4-nitrophenyl-alpha-L-arabinofuranoside wild-type, pH 7.0, 25°C Selenomonas ruminantium
4.38
-
 4-nitrophenyl-alpha-L-arabinofuranoside wild-type, pH 6.0, 25°C Selenomonas ruminantium
7.13
-
 4-nitrophenyl-beta-D-xylopyranoside wild-type, pH 8.0, 25°C Selenomonas ruminantium
36.4
-
 4-nitrophenyl-beta-D-xylopyranoside wild-type, pH 7.0, 25°C Selenomonas ruminantium
48.9
-
 4-nitrophenyl-beta-D-xylopyranoside wild-type, pH 6.0, 25°C Selenomonas ruminantium