Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.37 extracted from

  • Eneyskaya, E.V.; Ivanen, D.R.; Bobrov, K.S.; Isaeva-Ivanova, L.S.; Shabalin, K.A.; Savelev, A.N.; Golubev, A.M.; Kulminskaya, A.A.
    Biochemical and kinetic analysis of the GH3 family beta-xylosidase from Aspergillus awamori X-100 (2007), Arch. Biochem. Biophys., 457, 225-234.
    View publication on PubMed
Search for more literature for 3.2.1.37

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.25
-
 4-nitrophenyl-beta-D-xylopyranoside pH 4.5, 37°C Aspergillus awamori
6.8
-
 4-nitrophenyl-alpha-L-arabinofuranoside pH 4.5, 37°C Aspergillus awamori
55
-
 4-nitrophenyl-beta-D-glucopyranoside pH 4.5, 37°C Aspergillus awamori
64
-
 4-nitrophenyl-beta-D-fucopyranoside pH 4.5, 37°C Aspergillus awamori

Organism

Organism UniProt Comment Textmining
Aspergillus awamori
-
-
-
Aspergillus awamori X-100
-
-
-

Reaction

Reaction Comment Organism Reaction ID
(Xylbeta(1-4))x + H2O = beta-D-xylopyranose + (Xylbeta(1-4))x-1 hydrolysis of 4-nitrophenyl-beta-D-xylopyranoside with overall retention of substrate anomeric configuration, double-displacement mechanism. Deglycosylation is the rate-limiting step for the enzymatic hydrolysis Aspergillus awamori
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl alpha-L-arabinofuranoside + H2O
-
 Aspergillus awamori 4-nitrophenol + alpha-L-arabinofuranose
-
 ?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
-
 Aspergillus awamori X-100 4-nitrophenol + alpha-L-arabinofuranose
-
 ?
4-nitrophenyl beta-D-fucopyranoside + H2O
-
 Aspergillus awamori 4-nitrophenol + beta-D-fucose
-
 ?
4-nitrophenyl beta-D-fucopyranoside + H2O
-
 Aspergillus awamori X-100 4-nitrophenol + beta-D-fucose
-
 ?
4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Aspergillus awamori 4-nitrophenol + beta-D-glucose
-
 ?
4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Aspergillus awamori X-100 4-nitrophenol + beta-D-glucose
-
 ?
4-nitrophenyl beta-D-xylopyranoside + H2O hydrolysis with overall retention of substrate anomeric configuration Aspergillus awamori 4-nitrophenol + beta-D-xylopyranose
-
 ?
4-nitrophenyl beta-D-xylopyranoside + H2O hydrolysis with overall retention of substrate anomeric configuration Aspergillus awamori X-100 4-nitrophenol + beta-D-xylopyranose
-
 ?
additional information no substrate: 4-nitrophenyl beta-D-galactopyranoside, 4-nitrophenyl-alpha-D-glucopyranoside, 4-nitrophenyl-beta-D-xylopyranoside Aspergillus awamori ?
-
 ?
additional information no substrate: 4-nitrophenyl beta-D-galactopyranoside, 4-nitrophenyl-alpha-D-glucopyranoside, 4-nitrophenyl-beta-D-xylopyranoside Aspergillus awamori X-100 ?
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.001
-
 4-nitrophenyl-beta-D-fucopyranoside pH 4.5, 37°C Aspergillus awamori
0.24
-
 4-nitrophenyl-beta-D-glucopyranoside pH 4.5, 37°C Aspergillus awamori
17.5
-
 4-nitrophenyl-beta-D-xylopyranoside pH 4.5, 37°C Aspergillus awamori
31.5
-
 4-nitrophenyl-alpha-L-arabinofuranoside pH 4.5, 37°C Aspergillus awamori