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Literature summary for 3.2.1.37 extracted from

  • Morana, A.; Paris, O.; Maurelli, L.; Rossi, M.; Cannio, R.
    Gene cloning and expression in Escherichia coli of a bi-functional beta-D-xylosidase/alpha-L-arabinosidase from Sulfolobus solfataricus involved in xylan degradation (2007), Extremophiles, 11, 123-132.
    View publication on PubMed
Search for more literature for 3.2.1.37

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Saccharolobus solfataricus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
82000
-
 4 * 82000, SDS-PAGE Saccharolobus solfataricus
338800
-
 gel filtration Saccharolobus solfataricus

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus
-
 bi-functional beta-D-xylosidase/alpha-L-arabinosidase
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein Saccharolobus solfataricus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.63
-
 pH 6.5, 75°C Saccharolobus solfataricus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-nitrophenyl beta-D-glucopyranoside + H2O 1.7% of the activity with 4-nitrophenyl-alpha-L-arabinofuranoside Saccharolobus solfataricus 2-nitrophenol + beta-D-glucose
-
 ?
2-nitrophenyl beta-D-glucopyranoside + H2O 1.7% of the activity with 4-nitrophenyl-alpha-L-arabinofuranoside Saccharolobus solfataricus P2 2-nitrophenol + beta-D-glucose
-
 ?
2-nitrophenyl beta-D-xylopyranoside + H2O 3% of the activity with 4-nitrophenyl-alpha-L-arabinofuranoside Saccharolobus solfataricus 2-nitrophenol + D-xylose
-
 ?
2-nitrophenyl beta-D-xylopyranoside + H2O 3% of the activity with 4-nitrophenyl-alpha-L-arabinofuranoside Saccharolobus solfataricus P2 2-nitrophenol + D-xylose
-
 ?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
-
 Saccharolobus solfataricus 4-nitrophenol + alpha-L-arabinofuranose
-
 ?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
-
 Saccharolobus solfataricus P2 4-nitrophenol + alpha-L-arabinofuranose
-
 ?
4-nitrophenyl beta-D-glucopyranoside + H2O 9% of the activity with 4-nitrophenyl-alpha-L-arabinofuranoside Saccharolobus solfataricus 4-nitrophenol + beta-D-glucose
-
 ?
4-nitrophenyl beta-D-glucopyranoside + H2O 9% of the activity with 4-nitrophenyl-alpha-L-arabinofuranoside Saccharolobus solfataricus P2 4-nitrophenol + beta-D-glucose
-
 ?
4-nitrophenyl beta-D-xylopyranoside + H2O 14% of the activity with 4-nitrophenyl-alpha-L-arabinofuranoside Saccharolobus solfataricus 4-nitrophenol + beta-D-xylose
-
 ?
4-nitrophenyl beta-D-xylopyranoside + H2O 14% of the activity with 4-nitrophenyl-alpha-L-arabinofuranoside Saccharolobus solfataricus P2 4-nitrophenol + beta-D-xylose
-
 ?
4-nitrophenyl-alpha-L-arabinopyranoside + H2O 46% of the activity with 4-nitrophenyl-alpha-L-arabinofuranoside Saccharolobus solfataricus 4-nitrophenol + alpha-L-arabinose
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 82000, SDS-PAGE Saccharolobus solfataricus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
80 85 beta-D-xylosidase activity Saccharolobus solfataricus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80
-
 24 h, stable Saccharolobus solfataricus
90
-
 half-life 10.5 h Saccharolobus solfataricus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
 both beta-D-xylosidase and alpha-L-arabinosidase activity Saccharolobus solfataricus