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Literature summary for 3.2.1.37 extracted from

  • Minic, Z.; Rihouey, C.; Do, C.T.; Lerouge, P.; Jouanin, L.
    Purification and characterization of enzymes exhibiting beta-D-xylosidase activities in stem tissues of Arabidopsis (2004), Plant Physiol., 135, 867-878.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.37

Cloned(Commentary)

Cloned (Comment) Organism
gene xyl1, DNA and amino acid sequence determination and analysis, expression and phylogenetic analysis Arabidopsis thaliana
gene xyl4, DNA and amino acid sequence determination and analysis, expression and phylogenetic analysis Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.7
-
 4-nitrophenyl-beta-D-xylopyranoside pH 5.0, 37°C Arabidopsis thaliana
1.2
-
 4-nitrophenyl-beta-D-xylopyranoside pH 5.0, 37°C Arabidopsis thaliana

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
64000
-
 x * 64000, SDS-PAGE Arabidopsis thaliana
67000
-
 x * 67000, SDS-PAGE Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q9FGY1
-
-
Arabidopsis thaliana Q9FLG1
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein binds to concanavalin A Arabidopsis thaliana

Purification (Commentary)

Purification (Comment) Organism
native enzyme from stem, 29.1fold by concanavalin A affinity chromatography, cation exchange chromatography, and gel filtration to homogeneity Arabidopsis thaliana
native enzyme from stem, 40.3fold by concanavalin A affinity chromatography, cation exchange chromatography, and gel filtration to homogeneity Arabidopsis thaliana

Source Tissue

Source Tissue Comment Organism Textmining
stem
-
 Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl alpha-L-arabinofuranoside + H2O
-
 Arabidopsis thaliana 4-nitrophenol + alpha-L-arabinofuranose
-
 ?
4-nitrophenyl beta-D-xylopyranoside + H2O
-
 Arabidopsis thaliana 4-nitrophenol + beta-D-xylose
-
 ?
additional information xylotriose is a poor substrate Arabidopsis thaliana ?
-
 ?
additional information xylotriose is a poor substrate, the bifunctional enzyme shows two catalytic activities: alpha-L-arabinofuranosidase and beta-D-xylosidase activities Arabidopsis thaliana ?
-
 ?
xylobiose + H2O
-
 Arabidopsis thaliana 2 beta-D-xylose
-
 ?
xylotetraose + H2O
-
 Arabidopsis thaliana xylotriose + beta-D-xylose
-
 ?

Subunits

Subunits Comment Organism
? x * 67000, SDS-PAGE Arabidopsis thaliana
? x * 64000, SDS-PAGE Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
alpha-arabinofuranosidase/beta-xylosidase
-
 Arabidopsis thaliana
beta-D-xylosidase
-
 Arabidopsis thaliana
More cf. EC 3.2.1.55, alpha-L-arabinofuranosidase Arabidopsis thaliana
XYL1
-
 Arabidopsis thaliana
XYL4
-
 Arabidopsis thaliana
xylosidase
-
 Arabidopsis thaliana

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
 Arabidopsis thaliana
60
-
-
 Arabidopsis thaliana

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 70
-
 Arabidopsis thaliana

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.35
-
 4-nitrophenyl-beta-D-xylopyranoside pH 5.0, 37°C Arabidopsis thaliana
1.65
-
 4-nitrophenyl-beta-D-xylopyranoside pH 5.0, 37°C Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
 assay at Arabidopsis thaliana