Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | strong activation, and has a slight thermostabilizing effect | Mycothermus thermophilus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | complete inhibition at 1 mM | Mycothermus thermophilus | |
EDTA | 20% inhibition at 1 mM | Mycothermus thermophilus | |
Hg2+ | 91% inhibition at 1 mM | Mycothermus thermophilus | |
additional information | the enzyme is very tolerant to inhibition by D-xylose and is not affected up to 200 mM D-xylose | Mycothermus thermophilus | |
Zn2+ | 22% inhibition at 1 mM | Mycothermus thermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.5 | - |
4-nitrophenyl-beta-D-glucopyranoside | pH 5.0, 60°C | Mycothermus thermophilus | |
1.3 | - |
4-nitrophenyl-beta-D-xylopyranoside | pH 5.0, 60°C | Mycothermus thermophilus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme is not affected by Mg2+, Al3+, Co2+, Mn2+, Cu2+, K+, Ba2+, and Fe2+ | Mycothermus thermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycothermus thermophilus | - |
- |
- |
Mycothermus thermophilus 77.8 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme 125fold from mycelium by heat treatment at 50°C, ammonium sulfate fractionation, gel filtration, and ion exchange chromatography | Mycothermus thermophilus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mycelium | - |
Mycothermus thermophilus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
65 | - |
purified enzyme | Mycothermus thermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Mycothermus thermophilus | 4-nitrophenol + beta-D-glucose | - |
? | |
4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Mycothermus thermophilus 77.8 | 4-nitrophenol + beta-D-glucose | - |
? | |
4-nitrophenyl beta-D-xylopyranoside + H2O | specific for | Mycothermus thermophilus | 4-nitrophenol + beta-D-xylose | - |
? | |
4-nitrophenyl beta-D-xylopyranoside + H2O | specific for | Mycothermus thermophilus 77.8 | 4-nitrophenol + beta-D-xylose | - |
? | |
additional information | specific enzyme, no activity with 4-nitrophenyl-beta-D-galactopyranoside, 4-nitrophenyl-alpha-L-arabinopyranoside, 2-nitrophenyl-beta-D-galactopyranoside, carboxymethyl-cellulose, avicel, or xylan | Mycothermus thermophilus | ? | - |
? | |
additional information | specific enzyme, no activity with 4-nitrophenyl-beta-D-galactopyranoside, 4-nitrophenyl-alpha-L-arabinopyranoside, 2-nitrophenyl-beta-D-galactopyranoside, carboxymethyl-cellulose, avicel, or xylan | Mycothermus thermophilus 77.8 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
beta-D-xylosidase | - |
Mycothermus thermophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Mycothermus thermophilus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 75 | about 20% of maximal activity at 40°C, 50% at 75°C | Mycothermus thermophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
Ca2+ has a slight thermostabilizing effect | Mycothermus thermophilus |
50 | 60 | 60 min, fully stable | Mycothermus thermophilus |
65 | - |
half-life is 11 min in absence of Ca2+, half-life is 30 min in presence of Ca2+ | Mycothermus thermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Mycothermus thermophilus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.5 | 7.5 | the activity drops sharply below pH 4.5 and above pH 7.0 | Mycothermus thermophilus |