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Literature summary for 3.2.1.37 extracted from

  • Czjzek, M.; Bravman, T.; Henrissat, B.; Shoham, Y.
    Crystallization and preliminary crystallographic analysis of a thermostable family 52 beta-D-xylosidase from Geobacillus stearothermophilus T-6 (2004), Acta Crystallogr. Sect. D, D60, 1461-1463.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene xynB2, overexpression of the soluble enzyme in Escherichia coli strain BL21(DE3) Geobacillus stearothermophilus

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant enzyme, hanging drop vapour diffusion method, 0.002 ml protein solution containing 5 mg/ml protein in 50 mM Tris-HCl, pH 7.0, 100 mM NaCl, and 0.02% NaN3, is mixed with 0.002 ml well solution containing 2.3-2.4 M ammonium sulfate, and 40-60 mM sodium citrate at pH 5.5, a few weeks, X-ray diffraction structure determination and analysis at 2.0 A resolution Geobacillus stearothermophilus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
79894
-
2 * 79894, sequence calculation and crystal structure Geobacillus stearothermophilus

Organism

Organism UniProt Comment Textmining
Geobacillus stearothermophilus Q09LZ0 i.e. Bacillus stearothermophilus, strain T-6
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Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from Escherichia coli strain BL21(DE3) to near homogeneity Geobacillus stearothermophilus

Subunits

Subunits Comment Organism
dimer 2 * 79894, sequence calculation and crystal structure Geobacillus stearothermophilus

Synonyms

Synonyms Comment Organism
beta-D-xylosidase
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Geobacillus stearothermophilus
More the enzyme belongs to the glycoside hydrolase family 52 Geobacillus stearothermophilus
XynB2
-
Geobacillus stearothermophilus