Cloned (Comment) | Organism |
---|---|
gene xynB2, overexpression of the soluble enzyme in Escherichia coli strain BL21(DE3) | Geobacillus stearothermophilus |
Crystallization (Comment) | Organism |
---|---|
purified recombinant enzyme, hanging drop vapour diffusion method, 0.002 ml protein solution containing 5 mg/ml protein in 50 mM Tris-HCl, pH 7.0, 100 mM NaCl, and 0.02% NaN3, is mixed with 0.002 ml well solution containing 2.3-2.4 M ammonium sulfate, and 40-60 mM sodium citrate at pH 5.5, a few weeks, X-ray diffraction structure determination and analysis at 2.0 A resolution | Geobacillus stearothermophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
79894 | - |
2 * 79894, sequence calculation and crystal structure | Geobacillus stearothermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | Q09LZ0 | i.e. Bacillus stearothermophilus, strain T-6 | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli strain BL21(DE3) to near homogeneity | Geobacillus stearothermophilus |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 79894, sequence calculation and crystal structure | Geobacillus stearothermophilus |
Synonyms | Comment | Organism |
---|---|---|
beta-D-xylosidase | - |
Geobacillus stearothermophilus |
More | the enzyme belongs to the glycoside hydrolase family 52 | Geobacillus stearothermophilus |
XynB2 | - |
Geobacillus stearothermophilus |