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Literature summary for 3.2.1.37 extracted from

  • Vocadlo, D.J.; Wicki, J.; Rupitz, K.; Withers, S.G.
    A case for reverse protonation: identification of Glu160 as an acid/base catalyst in Thermoanaerobacterium saccharolyticum beta-xylosidase and detailed kinetic analysis of a site-directed mutant (2002), Biochemistry, 41, 9736-9746.
    View publication on PubMed
Search for more literature for 3.2.1.37

Protein Variants

Protein Variants Comment Organism
E160A E160 is the acid-base catalyst Thermoanaerobacterium saccharolyticum

Inhibitors

Inhibitors Comment Organism Structure
benzyl 1-thio-beta-D-xylopyranoside
-
 Thermoanaerobacterium saccharolyticum
D-xylose
-
 Thermoanaerobacterium saccharolyticum
N-bromoacetyl-beta-D-xylopyranosylamine pseudo-first-order inactivation Thermoanaerobacterium saccharolyticum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
 2,5-dinitrophenyl-beta-D-xylopyranoside pH 5.5, 37°C Thermoanaerobacterium saccharolyticum
0.01
-
 3,4-dinitrophenyl-beta-D-xylopyranoside pH 5.5, 37°C Thermoanaerobacterium saccharolyticum
0.011
-
 2,5-dinitrophenyl-beta-D-xylopyranoside pH 5.5, 37°C Thermoanaerobacterium saccharolyticum
0.014
-
 3,4-dinitrophenyl-beta-D-xylopyranoside pH 5.5, 37°C Thermoanaerobacterium saccharolyticum
0.036
-
 p-nitrophenyl-beta-D-xylopyranoside pH 5.5, 37°C Thermoanaerobacterium saccharolyticum
0.046
-
 o-nitrophenyl-beta-D-xylopyranoside pH 5.5, 37°C Thermoanaerobacterium saccharolyticum
0.08
-
 o-nitrophenyl-beta-D-xylopyranoside pH 5.5, 37°C Thermoanaerobacterium saccharolyticum
0.14
-
 p-nitrophenyl-beta-D-xylopyranoside pH 5.5, 37°C Thermoanaerobacterium saccharolyticum
0.15
-
 m-nitrophenyl-beta-D-xylopyranoside pH 5.5, 37°C Thermoanaerobacterium saccharolyticum
4.9
-
 m-nitrophenyl-beta-D-xylopyranoside pH 5.5, 37°C Thermoanaerobacterium saccharolyticum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
59700
-
 x * 59700, protein with C-terminal His-tag, mass spectrometry Thermoanaerobacterium saccharolyticum

Organism

Organism UniProt Comment Textmining
Thermoanaerobacterium saccharolyticum
-
-
-

Reaction

Reaction Comment Organism Reaction ID
(Xylbeta(1-4))x + H2O = beta-D-xylopyranose + (Xylbeta(1-4))x-1 kinetics Thermoanaerobacterium saccharolyticum
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,5-dinitrophenyl-beta-D-xylopyranoside + H2O
-
 Thermoanaerobacterium saccharolyticum 2,5-dinitrophenol + D-xylopyranose
-
 ?
3,4-dinitrophenyl beta-D-xylopyranoside + H2O
-
 Thermoanaerobacterium saccharolyticum 3,4-dinitrophenol + beta-D-xylopyranose
-
 ?
m-nitrophenyl-beta-D-xylopyranoside + H2O
-
 Thermoanaerobacterium saccharolyticum m-nitrophenol + beta-D-xylopyranose
-
 ?
o-nitrophenyl-beta-D-xylopyranoside + H2O
-
 Thermoanaerobacterium saccharolyticum o-nitrophenol + beta-D-xylopyranose
-
 ?
p-nitrophenyl-beta-D-xylopyranoside + H2O
-
 Thermoanaerobacterium saccharolyticum p-nitrophenol + beta-D-xylopyranose
-
 ?

Subunits

Subunits Comment Organism
? x * 59700, protein with C-terminal His-tag, mass spectrometry Thermoanaerobacterium saccharolyticum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4
-
 p-nitrophenyl-beta-D-xylopyranoside pH 5.5, 37°C Thermoanaerobacterium saccharolyticum

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
5.3
-
 benzyl 1-thio-beta-D-xylopyranoside pH 5.5, 37°C Thermoanaerobacterium saccharolyticum
20
-
 D-xylose pH 5.5, 37°C Thermoanaerobacterium saccharolyticum