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Literature summary for 3.2.1.37 extracted from
- Purification and properties of beta-xylosidase from Penicillium wortmanni (1978), Can. J. Biochem., 56, 43-50.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| N-bromosuccinimide | strong inhibition | Penicillium wortmanni |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.12 | - |
p-nitrophenyl beta-D-xylopyranoside | - |
Penicillium wortmanni | |
| 0.15 | - |
2,4-dinitrophenyl beta-D-xylopyranoside | - |
Penicillium wortmanni | |
| 0.2 | - |
2,4-dinitrophenyl 1-thio-beta-D-xylopyranoside | - |
Penicillium wortmanni | |
| 0.3 | - |
n-pentyl beta-D-xylopyranoside | - |
Penicillium wortmanni | |
| 1.4 | - |
methyl beta-D-xylopyranoside | - |
Penicillium wortmanni | |
| 2.4 | - |
p-nitrophenyl alpha-L-arabinopyranoside | - |
Penicillium wortmanni | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Penicillium wortmanni | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 100000 | - |
gel filtration, sucrose density gradient centrifugation, SDS-PAGE | Penicillium wortmanni |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| xylan + H2O | Penicillium wortmanni | low activity | beta-D-xylose + ? | - |
? | |
| xylo-oligosaccharides + H2O | Penicillium wortmanni | operating by a double-inversion mechanism | beta-D-xylose + ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Penicillium wortmanni | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| to homogeneity | Penicillium wortmanni |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | synthesis induced by methyl-beta- or methyl-alpha-D-xylopyranoside | Penicillium wortmanni | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2,4-dinitrophenyl beta-D-xylopyranoside + H2O | - |
Penicillium wortmanni | 2,4-dinitrophenol + beta-D-xylopyranose | - |
? | |
| 2,4-dinitrophenyl-1-thio-beta-D-xylopyranoside + H2O | - |
Penicillium wortmanni | 2,4-dinitrophenol + 1-thio-beta-D-xylopyranose | - |
? | |
| methyl beta-D-xylopyranoside + H2O | - |
Penicillium wortmanni | methanol + beta-D-xylopyranose | - |
? | |
| n-pentyl beta-D-xylopyranoside + H2O | - |
Penicillium wortmanni | n-pentanol + beta-D-xylopyranose | - |
? | |
| p-nitrophenyl alpha-L-arabinopyranoside + H2O | - |
Penicillium wortmanni | p-nitrophenol + alpha-L-arabinopyranose | - |
? | |
| p-nitrophenyl beta-D-xylopyranoside + H2O | - |
Penicillium wortmanni | p-nitrophenol + beta-D-xylopyranose | - |
? | |
| phenyl beta-D-xylopyranoside + methanol | in the presence of 1 M methanol, transxylosylation activity | Penicillium wortmanni | methyl beta-D-xylopyranoside + phenol | - |
? | |
| xylan + H2O | low activity | Penicillium wortmanni | beta-D-xylose + ? | - |
? | |
| xylo-oligosaccharides + H2O | operating by a double-inversion mechanism | Penicillium wortmanni | beta-D-xylose + ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 100000, SDS-PAGE, gel filtration | Penicillium wortmanni |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 3.3 | 4 | - |
Penicillium wortmanni |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 6 | most stable within | Penicillium wortmanni |
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